High-throughput proteomics uncovers exercise training and type 2 diabetes-induced changes in human white adipose tissue
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High-throughput proteomics uncovers exercise training and type 2 diabetes-induced changes in human white adipose tissue. / Larsen, Jeppe Kjærgaard; Kruse, Rikke; Sahebekhtiari, Navid; Moreno-Justicia, Roger; Jorba, Gerard Gomez; Petersen, Maria H.; de Almeida, Martin E.; Ørtenblad, Niels; Deshmukh, Atul S.; Højlund, Kurt.
In: Science Advances, Vol. 9, No. 48, eadi7548, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High-throughput proteomics uncovers exercise training and type 2 diabetes-induced changes in human white adipose tissue
AU - Larsen, Jeppe Kjærgaard
AU - Kruse, Rikke
AU - Sahebekhtiari, Navid
AU - Moreno-Justicia, Roger
AU - Jorba, Gerard Gomez
AU - Petersen, Maria H.
AU - de Almeida, Martin E.
AU - Ørtenblad, Niels
AU - Deshmukh, Atul S.
AU - Højlund, Kurt
PY - 2023
Y1 - 2023
N2 - White adipose tissue (WAT) is important for metabolic homeostasis. We established the differential proteomic signatures of WAT in glucose-tolerant lean and obese individuals and patients with type 2 diabetes (T2D) and the response to 8 weeks of high-intensity interval training (HIIT). Using a high-throughput and reproducible mass spectrometry-based proteomics pipeline, we identified 3773 proteins and found that most regulated proteins displayed progression in markers of dysfunctional WAT from lean to obese to T2D individuals and were highly associated with clinical measures such as insulin sensitivity and HbA1c. We propose that these distinct markers could serve as potential clinical biomarkers. HIIT induced only minor changes in the WAT proteome. This included an increase in WAT ferritin levels independent of obesity and T2D, and WAT ferritin levels were strongly correlated with individual insulin sensitivity. Together, we report a proteomic signature of WAT related to obesity and T2D and highlight an unrecognized role of human WAT iron metabolism in exercise training adaptations.
AB - White adipose tissue (WAT) is important for metabolic homeostasis. We established the differential proteomic signatures of WAT in glucose-tolerant lean and obese individuals and patients with type 2 diabetes (T2D) and the response to 8 weeks of high-intensity interval training (HIIT). Using a high-throughput and reproducible mass spectrometry-based proteomics pipeline, we identified 3773 proteins and found that most regulated proteins displayed progression in markers of dysfunctional WAT from lean to obese to T2D individuals and were highly associated with clinical measures such as insulin sensitivity and HbA1c. We propose that these distinct markers could serve as potential clinical biomarkers. HIIT induced only minor changes in the WAT proteome. This included an increase in WAT ferritin levels independent of obesity and T2D, and WAT ferritin levels were strongly correlated with individual insulin sensitivity. Together, we report a proteomic signature of WAT related to obesity and T2D and highlight an unrecognized role of human WAT iron metabolism in exercise training adaptations.
U2 - 10.1126/sciadv.adi7548
DO - 10.1126/sciadv.adi7548
M3 - Journal article
C2 - 38019916
AN - SCOPUS:85178192974
VL - 9
JO - Science advances
JF - Science advances
SN - 2375-2548
IS - 48
M1 - eadi7548
ER -
ID: 378809986