Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism

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  • Julie Massart
  • Rasmus J O Sjögren
  • Brendan Egan
  • Christian Garde
  • Magnus Lindgren
  • Weifeng Gu
  • Duarte M S Ferreira
  • Mutsumi Katayama
  • Jorge L Ruas
  • Barrès, Romain
  • Donal J O'Gorman
  • Zierath, Juleen R
  • Anna Krook

Skeletal muscle is a highly adaptable tissue and remodels in response to exercise training. Using short RNA sequencing, we determine the miRNA profile of skeletal muscle from healthy male volunteers before and after a 14-day aerobic exercise training regime. Among the exercise training-responsive miRNAs identified, miR-19b-3p was selected for further validation. Overexpression of miR-19b-3p in human skeletal muscle cells increases insulin signaling, glucose uptake, and maximal oxygen consumption, recapitulating the adaptive response to aerobic exercise training. Overexpression of miR-19b-3p in mouse flexor digitorum brevis muscle enhances contraction-induced glucose uptake, indicating that miR-19b-3p exerts control on exercise training-induced adaptations in skeletal muscle. Potential targets of miR-19b-3p that are reduced after aerobic exercise training include KIF13A, MAPK6, RNF11, and VPS37A. Amongst these, RNF11 silencing potentiates glucose uptake in human skeletal muscle cells. Collectively, we identify miR-19b-3p as an aerobic exercise training-induced miRNA that regulates skeletal muscle glucose metabolism.

Original languageEnglish
Article number5948
JournalNature Communications
Issue number1
Number of pages13
Publication statusPublished - 2021

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© 2021. The Author(s).

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