Sequencing only
The Single-Cell Omics Platform offers service for sequencing of user-prepared libraries on NovaSeq X Plus (Illumina).
Guidelines
Please be aware of the following and if you have questions, please contact us here.
- Libraries should be compatible with Illumina sequencing platforms.
- Libraries should be of good quality without adaptor dimers.
- We recommend the use of unique dual indices (UDI) for Illumina sequencing.
- Sequencing pools can only be run on whole flow cells.
- The user should specify which sequencing kit and number of sequencing cycles to use.
- Measure library concentration using Qubit or qPCR quantification methods.
- Estimate library quality and size using a capillary electrophoresis method (Bioanalyzer or Tapestation). Make sure no adapter-dimer traces are visible.
- Calculate the library molarity (in nanomolar)
- Normalize then pool librariesaccording to protocols, shortly before delivery to SCOP.Please avoid long-term storage of library pool.
Sequencing service include:
- Sequencing on NovaSeq X
- FASTQ files
Not included:
- Normalization and pooling (the user is responsible for correct quantification and pooling)
- Troubleshooting of failed or suboptimal runs
SCOP does not guarantee run-performance for Sequencing-only projects.
Sequencing price varies depending on kit. If you would like us to provide a sequencing cost estimate, please contact us with information about the number of reads required for your libraries.
Please recognize and acknowledge SCOP’s contributions to the scientific improvements in your project. Being acknowledged in publications is very important as it serves as documentation for the SCOPS's value and performance.