Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle

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Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. / Kramer, Henning F.; Witczak, Carol A.; Fujii, Nobuharu; Jessen, Niels; Taylor, Eric B.; Arnolds, David E.; Sakamoto, Kei; Hirshman, Michael F.; Goodyear, Laurie J.

In: Diabetes, Vol. 55, No. 7, 01.07.2006, p. 2067-2076.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kramer, HF, Witczak, CA, Fujii, N, Jessen, N, Taylor, EB, Arnolds, DE, Sakamoto, K, Hirshman, MF & Goodyear, LJ 2006, 'Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle', Diabetes, vol. 55, no. 7, pp. 2067-2076. https://doi.org/10.2337/db06-0150

APA

Kramer, H. F., Witczak, C. A., Fujii, N., Jessen, N., Taylor, E. B., Arnolds, D. E., Sakamoto, K., Hirshman, M. F., & Goodyear, L. J. (2006). Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes, 55(7), 2067-2076. https://doi.org/10.2337/db06-0150

Vancouver

Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE et al. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes. 2006 Jul 1;55(7):2067-2076. https://doi.org/10.2337/db06-0150

Author

Kramer, Henning F. ; Witczak, Carol A. ; Fujii, Nobuharu ; Jessen, Niels ; Taylor, Eric B. ; Arnolds, David E. ; Sakamoto, Kei ; Hirshman, Michael F. ; Goodyear, Laurie J. / Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. In: Diabetes. 2006 ; Vol. 55, No. 7. pp. 2067-2076.

Bibtex

@article{af1f5977316d4116a32d925dd146391e,
title = "Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle",
abstract = "Insulin and contraction increase GLUT4 translocation in skeletal muscle via distinct signaling mechanisms. Akt substrate of 160 kDa (AS160) mediates insulin-stimulated GLUT4 translocation in L6 myotubes, presumably through activation of Akt. Using in vivo, in vitro, and in situ methods, insulin, contraction, and the AMP-activated protein kinase (AMPK) activator AICAR all increased AS160 phosphorylation in mouse skeletal muscle. Insulin-stimulated AS160 phosphorylation was fully blunted by wortmannin in vitro and in Akt2 knockout (KO) mice in vivo. In contrast, contraction-stimulated AS160 phosphorylation was only partially decreased by wortmannin and unaffected in Akt2 KO mice, suggesting additional regulatory mechanisms. To determine if AMPK mediates AS160 signaling, we used AMPK α2-inactive (α2i) transgenic mice. AICAR-stimulated AS160 phosphorylation was fully inhibited, whereas contraction-stimulated AS160 phosphorylation was partially reduced in the AMPK α2i transgenic mice. Combined AMPK α2 and Akt inhibition by wortmannin treatment of AMPK α2 transgenic mice did not fully ablate contraction-stimulated AS160 phosphorylation. Maximal insulin, together with either AICAR or contraction, increased AS160 phosphorylation in an additive manner. In conclusion, AS160 may be a point of convergence linking insulin, contraction, and AICAR signaling. While Akt and AMPK α2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation.",
keywords = "α2i, α2-inactive, AMPK, AMP-activated protein kinase, aPKC, atypical protein kinase C, AS160, Akt substrate of 160 kDa, EDL, extensor digitorum longus, GAP, GTPase-activating protein, PAS, phospho-Akt substrate",
author = "Kramer, {Henning F.} and Witczak, {Carol A.} and Nobuharu Fujii and Niels Jessen and Taylor, {Eric B.} and Arnolds, {David E.} and Kei Sakamoto and Hirshman, {Michael F.} and Goodyear, {Laurie J.}",
year = "2006",
month = jul,
day = "1",
doi = "10.2337/db06-0150",
language = "English",
volume = "55",
pages = "2067--2076",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "7",

}

RIS

TY - JOUR

T1 - Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle

AU - Kramer, Henning F.

AU - Witczak, Carol A.

AU - Fujii, Nobuharu

AU - Jessen, Niels

AU - Taylor, Eric B.

AU - Arnolds, David E.

AU - Sakamoto, Kei

AU - Hirshman, Michael F.

AU - Goodyear, Laurie J.

PY - 2006/7/1

Y1 - 2006/7/1

N2 - Insulin and contraction increase GLUT4 translocation in skeletal muscle via distinct signaling mechanisms. Akt substrate of 160 kDa (AS160) mediates insulin-stimulated GLUT4 translocation in L6 myotubes, presumably through activation of Akt. Using in vivo, in vitro, and in situ methods, insulin, contraction, and the AMP-activated protein kinase (AMPK) activator AICAR all increased AS160 phosphorylation in mouse skeletal muscle. Insulin-stimulated AS160 phosphorylation was fully blunted by wortmannin in vitro and in Akt2 knockout (KO) mice in vivo. In contrast, contraction-stimulated AS160 phosphorylation was only partially decreased by wortmannin and unaffected in Akt2 KO mice, suggesting additional regulatory mechanisms. To determine if AMPK mediates AS160 signaling, we used AMPK α2-inactive (α2i) transgenic mice. AICAR-stimulated AS160 phosphorylation was fully inhibited, whereas contraction-stimulated AS160 phosphorylation was partially reduced in the AMPK α2i transgenic mice. Combined AMPK α2 and Akt inhibition by wortmannin treatment of AMPK α2 transgenic mice did not fully ablate contraction-stimulated AS160 phosphorylation. Maximal insulin, together with either AICAR or contraction, increased AS160 phosphorylation in an additive manner. In conclusion, AS160 may be a point of convergence linking insulin, contraction, and AICAR signaling. While Akt and AMPK α2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation.

AB - Insulin and contraction increase GLUT4 translocation in skeletal muscle via distinct signaling mechanisms. Akt substrate of 160 kDa (AS160) mediates insulin-stimulated GLUT4 translocation in L6 myotubes, presumably through activation of Akt. Using in vivo, in vitro, and in situ methods, insulin, contraction, and the AMP-activated protein kinase (AMPK) activator AICAR all increased AS160 phosphorylation in mouse skeletal muscle. Insulin-stimulated AS160 phosphorylation was fully blunted by wortmannin in vitro and in Akt2 knockout (KO) mice in vivo. In contrast, contraction-stimulated AS160 phosphorylation was only partially decreased by wortmannin and unaffected in Akt2 KO mice, suggesting additional regulatory mechanisms. To determine if AMPK mediates AS160 signaling, we used AMPK α2-inactive (α2i) transgenic mice. AICAR-stimulated AS160 phosphorylation was fully inhibited, whereas contraction-stimulated AS160 phosphorylation was partially reduced in the AMPK α2i transgenic mice. Combined AMPK α2 and Akt inhibition by wortmannin treatment of AMPK α2 transgenic mice did not fully ablate contraction-stimulated AS160 phosphorylation. Maximal insulin, together with either AICAR or contraction, increased AS160 phosphorylation in an additive manner. In conclusion, AS160 may be a point of convergence linking insulin, contraction, and AICAR signaling. While Akt and AMPK α2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation.

KW - α2i, α2-inactive

KW - AMPK, AMP-activated protein kinase

KW - aPKC, atypical protein kinase C

KW - AS160, Akt substrate of 160 kDa

KW - EDL, extensor digitorum longus

KW - GAP, GTPase-activating protein

KW - PAS, phospho-Akt substrate

UR - http://www.scopus.com/inward/record.url?scp=33747039008&partnerID=8YFLogxK

U2 - 10.2337/db06-0150

DO - 10.2337/db06-0150

M3 - Journal article

C2 - 16804077

AN - SCOPUS:33747039008

VL - 55

SP - 2067

EP - 2076

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 7

ER -

ID: 239584833