Role of AMP-activated protein kinase in regulating hypoxic survival and proliferation of mesenchymal stem cells
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Role of AMP-activated protein kinase in regulating hypoxic survival and proliferation of mesenchymal stem cells. / De Meester, Carole; Timmermans, Aurélie D.; Balteau, Magali; Ginion, Audrey; Roelants, Véronique; Noppe, Gauthier; Porporato, Paolo E.; Sonveaux, Pierre; Viollet, Benoît; Sakamoto, Kei; Feron, Olivier; Horman, Sandrine; Vanoverschelde, Jean Louis; Beauloye, Christophe; Bertrand, Luc.
In: Cardiovascular Research, Vol. 101, No. 1, 01.01.2014, p. 20-29.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Role of AMP-activated protein kinase in regulating hypoxic survival and proliferation of mesenchymal stem cells
AU - De Meester, Carole
AU - Timmermans, Aurélie D.
AU - Balteau, Magali
AU - Ginion, Audrey
AU - Roelants, Véronique
AU - Noppe, Gauthier
AU - Porporato, Paolo E.
AU - Sonveaux, Pierre
AU - Viollet, Benoît
AU - Sakamoto, Kei
AU - Feron, Olivier
AU - Horman, Sandrine
AU - Vanoverschelde, Jean Louis
AU - Beauloye, Christophe
AU - Bertrand, Luc
PY - 2014/1/1
Y1 - 2014/1/1
N2 - AimsMesenchymal stem cells (MSCs) are widely used for cell therapy, particularly for the treatment of ischaemic heart disease. Mechanisms underlying control of their metabolism and proliferation capacity, critical elements for their survival and differentiation, have not been fully characterized. AMP-activated protein kinase (AMPK) is a key regulator known to metabolically protect cardiomyocytes against ischaemic injuries and, more generally, to inhibit cell proliferation. We hypothesized that AMPK plays a role in control of MSC metabolism and proliferation.Methods and resultsMSCs isolated from murine bone marrow exclusively expressed the AMPK1 catalytic subunit. In contrast to cardiomyocytes, a chronic exposure of MSCs to hypoxia failed to induce cell death despite the absence of AMPK activation. This hypoxic tolerance was the consequence of a preference of MSC towards glycolytic metabolism independently of oxygen availability and AMPK signalling. On the other hand, A-769662, a well-characterized AMPK activator, was able to induce a robust and sustained AMPK activation. We showed that A-769662-induced AMPK activation inhibited MSC proliferation. Proliferation was not arrested in MSCs derived from AMPK1-knockout mice, providing genetic evidence that AMPK is essential for this process. Among AMPK downstream targets proposed to regulate cell proliferation, we showed that neither the p70 ribosomal S6 protein kinase/eukaryotic elongation factor 2-dependent protein synthesis pathway nor p21 was involved, whereas p27 expression was increased by A-769662. Silencing p27 expression partially prevented the A-769662-dependent inhibition of MSC proliferation.ConclusionMSCs resist hypoxia independently of AMPK whereas chronic AMPK activation inhibits MSC proliferation, p27 being involved in this regulation.
AB - AimsMesenchymal stem cells (MSCs) are widely used for cell therapy, particularly for the treatment of ischaemic heart disease. Mechanisms underlying control of their metabolism and proliferation capacity, critical elements for their survival and differentiation, have not been fully characterized. AMP-activated protein kinase (AMPK) is a key regulator known to metabolically protect cardiomyocytes against ischaemic injuries and, more generally, to inhibit cell proliferation. We hypothesized that AMPK plays a role in control of MSC metabolism and proliferation.Methods and resultsMSCs isolated from murine bone marrow exclusively expressed the AMPK1 catalytic subunit. In contrast to cardiomyocytes, a chronic exposure of MSCs to hypoxia failed to induce cell death despite the absence of AMPK activation. This hypoxic tolerance was the consequence of a preference of MSC towards glycolytic metabolism independently of oxygen availability and AMPK signalling. On the other hand, A-769662, a well-characterized AMPK activator, was able to induce a robust and sustained AMPK activation. We showed that A-769662-induced AMPK activation inhibited MSC proliferation. Proliferation was not arrested in MSCs derived from AMPK1-knockout mice, providing genetic evidence that AMPK is essential for this process. Among AMPK downstream targets proposed to regulate cell proliferation, we showed that neither the p70 ribosomal S6 protein kinase/eukaryotic elongation factor 2-dependent protein synthesis pathway nor p21 was involved, whereas p27 expression was increased by A-769662. Silencing p27 expression partially prevented the A-769662-dependent inhibition of MSC proliferation.ConclusionMSCs resist hypoxia independently of AMPK whereas chronic AMPK activation inhibits MSC proliferation, p27 being involved in this regulation.
KW - AMPK
KW - Glycolysis
KW - Hypoxia
KW - Mesenchymal stem cells
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=84891352333&partnerID=8YFLogxK
U2 - 10.1093/cvr/cvt227
DO - 10.1093/cvr/cvt227
M3 - Journal article
C2 - 24104879
AN - SCOPUS:84891352333
VL - 101
SP - 20
EP - 29
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 1
ER -
ID: 239216035