Lipidomics is the large-scale study of pathways and networks of cellular lipids in biological systems. Analysis of lipids demands specific extraction methods. The majority lipid extraction and isolation methods from biological samples use the high solubility of non-polar chains in organic solvents. Most commonly used methods involves chloroform/methanol-based protocols with phase partitioning the lipids into the organic layer. However, several other protocols now exists. Although most lipidomics extraction protocols work well for a large variety of lipids, extraction methods might be necessary to be adapted for lipid species with specific properties and that are low abundant and labile. Similar to metabolomics analysis, with the diversity in lipid classes, it is not possible to accommodate all classes with a common extraction method.
With our lipidomics methodology we can detect in human plasma e.g. following lipid classes: triglycerides, diglycerides, Cholesteryl esters, phoshatidylcholines, phosphatatidylethanolamines, lyso-PCs, Lyso-PEs, Lyso-PIs, Spingomyelins, ceramides and fatty acids.