Impact of Antibiotic Treatment on the Gut Microbiome and its Resistome in Hematopoietic Stem Cell Transplant Recipients
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Impact of Antibiotic Treatment on the Gut Microbiome and its Resistome in Hematopoietic Stem Cell Transplant Recipients. / Nørgaard, Jens Christian; Jørgensen, Mette; Moestrup, Kasper Sommerlund; Ilett, Emma Elizabeth; Zucco, Adrian Gabriel; Marandi, Ramtin Z; Julian, Marc Noguera; Paredes, Roger; Lundgren, Jens D; Sengeløv, Henrik; MacPherson, Cameron Ross.
In: The Journal of Infectious Diseases, Vol. 228, No. 1, 2023, p. 28-36.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Impact of Antibiotic Treatment on the Gut Microbiome and its Resistome in Hematopoietic Stem Cell Transplant Recipients
AU - Nørgaard, Jens Christian
AU - Jørgensen, Mette
AU - Moestrup, Kasper Sommerlund
AU - Ilett, Emma Elizabeth
AU - Zucco, Adrian Gabriel
AU - Marandi, Ramtin Z
AU - Julian, Marc Noguera
AU - Paredes, Roger
AU - Lundgren, Jens D
AU - Sengeløv, Henrik
AU - MacPherson, Cameron Ross
N1 - © The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2023
Y1 - 2023
N2 - Antibiotic-resistant bacterial infections are increasingly an issue in allogenic hematopoietic stem cell transplant patients. How antibiotic treatment impacts antibiotic resistance in the human gut microbiome remains poorly understood in vivo. Here, a total of 577 fecal samples from 233 heavily antibiotic-treated transplant patients were examined using high-resolution prescription data and shotgun metagenomics. The 13 most frequently used antibiotics were significantly associated with 154 (40% of tested associations) microbiome features. Use of broad-spectrum β-lactam antibiotics was most markedly associated with microbial disruption and increase in resistome features. The enterococcal vanA gene was positively associated with 8 of the 13 antibiotics, and in particular piperacillin/tazobactam and vancomycin. Here, we highlight the need for a high-resolution approach in understanding the development of antibiotic resistance in the gut microbiome. Our findings can be used to inform antibiotic stewardship and combat the increasing threat of antibiotic resistance.
AB - Antibiotic-resistant bacterial infections are increasingly an issue in allogenic hematopoietic stem cell transplant patients. How antibiotic treatment impacts antibiotic resistance in the human gut microbiome remains poorly understood in vivo. Here, a total of 577 fecal samples from 233 heavily antibiotic-treated transplant patients were examined using high-resolution prescription data and shotgun metagenomics. The 13 most frequently used antibiotics were significantly associated with 154 (40% of tested associations) microbiome features. Use of broad-spectrum β-lactam antibiotics was most markedly associated with microbial disruption and increase in resistome features. The enterococcal vanA gene was positively associated with 8 of the 13 antibiotics, and in particular piperacillin/tazobactam and vancomycin. Here, we highlight the need for a high-resolution approach in understanding the development of antibiotic resistance in the gut microbiome. Our findings can be used to inform antibiotic stewardship and combat the increasing threat of antibiotic resistance.
KW - Humans
KW - Gastrointestinal Microbiome/genetics
KW - Anti-Bacterial Agents/adverse effects
KW - Drug Resistance, Microbial/genetics
KW - Bacteria/genetics
KW - Hematopoietic Stem Cell Transplantation/adverse effects
U2 - 10.1093/infdis/jiad033
DO - 10.1093/infdis/jiad033
M3 - Journal article
C2 - 36751730
VL - 228
SP - 28
EP - 36
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 1
ER -
ID: 358555227