Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. / Giannakis, Marios; Stappenbeck, Thaddeus S; Mills, Jason C; Leip, Douglas G; Lovett, Michael; Clifton, Sandra W; Ippolito, Joseph E; Glasscock, Jarret I; Arumugam, Manimozhiyan; Brent, Michael R; Gordon, Jeffrey I.

In: Journal of Biological Chemistry, Vol. 281, No. 16, 2006, p. 11292-300.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Giannakis, M, Stappenbeck, TS, Mills, JC, Leip, DG, Lovett, M, Clifton, SW, Ippolito, JE, Glasscock, JI, Arumugam, M, Brent, MR & Gordon, JI 2006, 'Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches', Journal of Biological Chemistry, vol. 281, no. 16, pp. 11292-300. https://doi.org/10.1074/jbc.M512118200

APA

Giannakis, M., Stappenbeck, T. S., Mills, J. C., Leip, D. G., Lovett, M., Clifton, S. W., Ippolito, J. E., Glasscock, J. I., Arumugam, M., Brent, M. R., & Gordon, J. I. (2006). Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. Journal of Biological Chemistry, 281(16), 11292-300. https://doi.org/10.1074/jbc.M512118200

Vancouver

Giannakis M, Stappenbeck TS, Mills JC, Leip DG, Lovett M, Clifton SW et al. Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. Journal of Biological Chemistry. 2006;281(16):11292-300. https://doi.org/10.1074/jbc.M512118200

Author

Giannakis, Marios ; Stappenbeck, Thaddeus S ; Mills, Jason C ; Leip, Douglas G ; Lovett, Michael ; Clifton, Sandra W ; Ippolito, Joseph E ; Glasscock, Jarret I ; Arumugam, Manimozhiyan ; Brent, Michael R ; Gordon, Jeffrey I. / Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 16. pp. 11292-300.

Bibtex

@article{6c963969055e426f9dfd2e2935c1218d,
title = "Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches",
abstract = "We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.",
keywords = "Animals, Computational Biology, DNA, Complementary, Epithelial Cells, Epithelium, Expressed Sequence Tags, Gastric Mucosa, Gene Library, Genome, Hematopoietic Stem Cells, Immunohistochemistry, Intestine, Small, Intestines, Lasers, Mice, Models, Biological, Neurons, RNA, Messenger, Signal Transduction, Software, Stem Cells, Stomach, Transcription Factors",
author = "Marios Giannakis and Stappenbeck, {Thaddeus S} and Mills, {Jason C} and Leip, {Douglas G} and Michael Lovett and Clifton, {Sandra W} and Ippolito, {Joseph E} and Glasscock, {Jarret I} and Manimozhiyan Arumugam and Brent, {Michael R} and Gordon, {Jeffrey I}",
year = "2006",
doi = "10.1074/jbc.M512118200",
language = "English",
volume = "281",
pages = "11292--300",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "16",

}

RIS

TY - JOUR

T1 - Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches

AU - Giannakis, Marios

AU - Stappenbeck, Thaddeus S

AU - Mills, Jason C

AU - Leip, Douglas G

AU - Lovett, Michael

AU - Clifton, Sandra W

AU - Ippolito, Joseph E

AU - Glasscock, Jarret I

AU - Arumugam, Manimozhiyan

AU - Brent, Michael R

AU - Gordon, Jeffrey I

PY - 2006

Y1 - 2006

N2 - We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.

AB - We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.

KW - Animals

KW - Computational Biology

KW - DNA, Complementary

KW - Epithelial Cells

KW - Epithelium

KW - Expressed Sequence Tags

KW - Gastric Mucosa

KW - Gene Library

KW - Genome

KW - Hematopoietic Stem Cells

KW - Immunohistochemistry

KW - Intestine, Small

KW - Intestines

KW - Lasers

KW - Mice

KW - Models, Biological

KW - Neurons

KW - RNA, Messenger

KW - Signal Transduction

KW - Software

KW - Stem Cells

KW - Stomach

KW - Transcription Factors

U2 - 10.1074/jbc.M512118200

DO - 10.1074/jbc.M512118200

M3 - Journal article

C2 - 16464855

VL - 281

SP - 11292

EP - 11300

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -

ID: 43976056