Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine. / Buhl, T; Thim, L; Kofod, Hans; Orskov, C; Harling, H; Holst, J J.

In: Journal of Biological Chemistry, Vol. 263, No. 18, 25.06.1988, p. 8621-4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Buhl, T, Thim, L, Kofod, H, Orskov, C, Harling, H & Holst, JJ 1988, 'Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine', Journal of Biological Chemistry, vol. 263, no. 18, pp. 8621-4.

APA

Buhl, T., Thim, L., Kofod, H., Orskov, C., Harling, H., & Holst, J. J. (1988). Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine. Journal of Biological Chemistry, 263(18), 8621-4.

Vancouver

Buhl T, Thim L, Kofod H, Orskov C, Harling H, Holst JJ. Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine. Journal of Biological Chemistry. 1988 Jun 25;263(18):8621-4.

Author

Buhl, T ; Thim, L ; Kofod, Hans ; Orskov, C ; Harling, H ; Holst, J J. / Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 18. pp. 8621-4.

Bibtex

@article{97936737a1574c6a900e83da10336622,
title = "Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine",
abstract = "Recent studies have revealed that the glucagon gene is expressed in the mammalian intestine. Here it codes for {"}glicentin{"} (proglucagon 1-69) and a glucagon-like peptide, proglucagon 78-107, recently isolated from porcine intestine. We studied the fate of the remaining COOH-terminal part of proglucagon (proglucagon 111-160) using radioimmunoassays against proglucagon 111-123 and 126-160. Two peptides were isolated from acid ethanol extracts of porcine ileal mucosa and sequenced: one corresponding to proglucagon 126-158 and one probably corresponding to proglucagon 111-158. By comparing human and porcine proglucagon sequences, Ala117 is replaced by Thr, and Ile138, Ala144, Ile152 and Gln153 are replaced by Val, Thr, Leu, and His. By gel filtration and radioimmunoassay of intestinal extracts it was established that a large part of porcine and virtually all of human proglucagon are processed to release proglucagon 111-123 (designated spacer peptide 2), which, like proglucagon 126-158 must be considered a potential hormonal entity. By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide.",
keywords = "Amino Acid Sequence, Animals, Glucagon, Humans, Ileum, Intestinal Mucosa, Molecular Sequence Data, Peptide Fragments, Protein Precursors, Radioimmunoassay, Species Specificity, Swine",
author = "T Buhl and L Thim and Hans Kofod and C Orskov and H Harling and Holst, {J J}",
year = "1988",
month = jun,
day = "25",
language = "English",
volume = "263",
pages = "8621--4",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "18",

}

RIS

TY - JOUR

T1 - Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine

AU - Buhl, T

AU - Thim, L

AU - Kofod, Hans

AU - Orskov, C

AU - Harling, H

AU - Holst, J J

PY - 1988/6/25

Y1 - 1988/6/25

N2 - Recent studies have revealed that the glucagon gene is expressed in the mammalian intestine. Here it codes for "glicentin" (proglucagon 1-69) and a glucagon-like peptide, proglucagon 78-107, recently isolated from porcine intestine. We studied the fate of the remaining COOH-terminal part of proglucagon (proglucagon 111-160) using radioimmunoassays against proglucagon 111-123 and 126-160. Two peptides were isolated from acid ethanol extracts of porcine ileal mucosa and sequenced: one corresponding to proglucagon 126-158 and one probably corresponding to proglucagon 111-158. By comparing human and porcine proglucagon sequences, Ala117 is replaced by Thr, and Ile138, Ala144, Ile152 and Gln153 are replaced by Val, Thr, Leu, and His. By gel filtration and radioimmunoassay of intestinal extracts it was established that a large part of porcine and virtually all of human proglucagon are processed to release proglucagon 111-123 (designated spacer peptide 2), which, like proglucagon 126-158 must be considered a potential hormonal entity. By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide.

AB - Recent studies have revealed that the glucagon gene is expressed in the mammalian intestine. Here it codes for "glicentin" (proglucagon 1-69) and a glucagon-like peptide, proglucagon 78-107, recently isolated from porcine intestine. We studied the fate of the remaining COOH-terminal part of proglucagon (proglucagon 111-160) using radioimmunoassays against proglucagon 111-123 and 126-160. Two peptides were isolated from acid ethanol extracts of porcine ileal mucosa and sequenced: one corresponding to proglucagon 126-158 and one probably corresponding to proglucagon 111-158. By comparing human and porcine proglucagon sequences, Ala117 is replaced by Thr, and Ile138, Ala144, Ile152 and Gln153 are replaced by Val, Thr, Leu, and His. By gel filtration and radioimmunoassay of intestinal extracts it was established that a large part of porcine and virtually all of human proglucagon are processed to release proglucagon 111-123 (designated spacer peptide 2), which, like proglucagon 126-158 must be considered a potential hormonal entity. By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide.

KW - Amino Acid Sequence

KW - Animals

KW - Glucagon

KW - Humans

KW - Ileum

KW - Intestinal Mucosa

KW - Molecular Sequence Data

KW - Peptide Fragments

KW - Protein Precursors

KW - Radioimmunoassay

KW - Species Specificity

KW - Swine

M3 - Journal article

C2 - 3379036

VL - 263

SP - 8621

EP - 8624

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 18

ER -

ID: 45575151