NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program. / Hiraike, Yuta; Waki, Hironori; Yu, Jing; Nakamura, Masahiro; Miyake, Kana; Nagano, Gaku; Nakaki, Ryo; Suzuki, Ken; Kobayashi, Hirofumi; Yamamoto, Shogo; Sun, Wei; Aoyama, Tomohisa; Hirota, Yusuke; Ohno, Haruya; Oki, Kenji; Yoneda, Masayasu; White, Andrew P; Tseng, Yu-Hua; Cypess, Aaron M; Larsen, Therese J; Jespersen, Naja Z; Scheele, Camilla; Tsutsumi, Shuichi; Aburatani, Hiroyuki; Yamauchi, Toshimasa; Kadowaki, Takashi.

In: Nature Cell Biology, Vol. 19, No. 9, 09.2017, p. 1081-1092.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hiraike, Y, Waki, H, Yu, J, Nakamura, M, Miyake, K, Nagano, G, Nakaki, R, Suzuki, K, Kobayashi, H, Yamamoto, S, Sun, W, Aoyama, T, Hirota, Y, Ohno, H, Oki, K, Yoneda, M, White, AP, Tseng, Y-H, Cypess, AM, Larsen, TJ, Jespersen, NZ, Scheele, C, Tsutsumi, S, Aburatani, H, Yamauchi, T & Kadowaki, T 2017, 'NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program', Nature Cell Biology, vol. 19, no. 9, pp. 1081-1092. https://doi.org/10.1038/ncb3590

APA

Hiraike, Y., Waki, H., Yu, J., Nakamura, M., Miyake, K., Nagano, G., Nakaki, R., Suzuki, K., Kobayashi, H., Yamamoto, S., Sun, W., Aoyama, T., Hirota, Y., Ohno, H., Oki, K., Yoneda, M., White, A. P., Tseng, Y-H., Cypess, A. M., ... Kadowaki, T. (2017). NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program. Nature Cell Biology, 19(9), 1081-1092. https://doi.org/10.1038/ncb3590

Vancouver

Hiraike Y, Waki H, Yu J, Nakamura M, Miyake K, Nagano G et al. NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program. Nature Cell Biology. 2017 Sep;19(9):1081-1092. https://doi.org/10.1038/ncb3590

Author

Hiraike, Yuta ; Waki, Hironori ; Yu, Jing ; Nakamura, Masahiro ; Miyake, Kana ; Nagano, Gaku ; Nakaki, Ryo ; Suzuki, Ken ; Kobayashi, Hirofumi ; Yamamoto, Shogo ; Sun, Wei ; Aoyama, Tomohisa ; Hirota, Yusuke ; Ohno, Haruya ; Oki, Kenji ; Yoneda, Masayasu ; White, Andrew P ; Tseng, Yu-Hua ; Cypess, Aaron M ; Larsen, Therese J ; Jespersen, Naja Z ; Scheele, Camilla ; Tsutsumi, Shuichi ; Aburatani, Hiroyuki ; Yamauchi, Toshimasa ; Kadowaki, Takashi. / NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program. In: Nature Cell Biology. 2017 ; Vol. 19, No. 9. pp. 1081-1092.

Bibtex

@article{eb0ab19a0a48413b9a8ed2d0b299c460,
title = "NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program",
abstract = "Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARγ to control the brown fat gene program.",
keywords = "Journal Article",
author = "Yuta Hiraike and Hironori Waki and Jing Yu and Masahiro Nakamura and Kana Miyake and Gaku Nagano and Ryo Nakaki and Ken Suzuki and Hirofumi Kobayashi and Shogo Yamamoto and Wei Sun and Tomohisa Aoyama and Yusuke Hirota and Haruya Ohno and Kenji Oki and Masayasu Yoneda and White, {Andrew P} and Yu-Hua Tseng and Cypess, {Aaron M} and Larsen, {Therese J} and Jespersen, {Naja Z} and Camilla Scheele and Shuichi Tsutsumi and Hiroyuki Aburatani and Toshimasa Yamauchi and Takashi Kadowaki",
year = "2017",
month = sep,
doi = "10.1038/ncb3590",
language = "English",
volume = "19",
pages = "1081--1092",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "nature publishing group",
number = "9",

}

RIS

TY - JOUR

T1 - NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program

AU - Hiraike, Yuta

AU - Waki, Hironori

AU - Yu, Jing

AU - Nakamura, Masahiro

AU - Miyake, Kana

AU - Nagano, Gaku

AU - Nakaki, Ryo

AU - Suzuki, Ken

AU - Kobayashi, Hirofumi

AU - Yamamoto, Shogo

AU - Sun, Wei

AU - Aoyama, Tomohisa

AU - Hirota, Yusuke

AU - Ohno, Haruya

AU - Oki, Kenji

AU - Yoneda, Masayasu

AU - White, Andrew P

AU - Tseng, Yu-Hua

AU - Cypess, Aaron M

AU - Larsen, Therese J

AU - Jespersen, Naja Z

AU - Scheele, Camilla

AU - Tsutsumi, Shuichi

AU - Aburatani, Hiroyuki

AU - Yamauchi, Toshimasa

AU - Kadowaki, Takashi

PY - 2017/9

Y1 - 2017/9

N2 - Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARγ to control the brown fat gene program.

AB - Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Finally, expression of NFIA and the brown-fat-specific genes is positively correlated in human brown fat. These results indicate that NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARγ to control the brown fat gene program.

KW - Journal Article

U2 - 10.1038/ncb3590

DO - 10.1038/ncb3590

M3 - Journal article

C2 - 28812581

VL - 19

SP - 1081

EP - 1092

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 9

ER -

ID: 182619023