Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice

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Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice. / Waget, Aurélie; Cabou, Cendrine; Masseboeuf, Myriam; Cattan, Pierre; Armanet, Mattieu; Karaca, Mélis; Castel, Julien; Garret, Celine; Payros, Gaëlle; Maida, Adriano; Sulpice, Thierry; Holst, Jens Juul; Drucker, Daniel J; Magnan, Christophe; Burcelin, Rémy.

In: Endocrinology, Vol. 152, No. 8, 08.2011, p. 3018-3029.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Waget, A, Cabou, C, Masseboeuf, M, Cattan, P, Armanet, M, Karaca, M, Castel, J, Garret, C, Payros, G, Maida, A, Sulpice, T, Holst, JJ, Drucker, DJ, Magnan, C & Burcelin, R 2011, 'Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice', Endocrinology, vol. 152, no. 8, pp. 3018-3029. https://doi.org/10.1210/en.2011-0286

APA

Waget, A., Cabou, C., Masseboeuf, M., Cattan, P., Armanet, M., Karaca, M., Castel, J., Garret, C., Payros, G., Maida, A., Sulpice, T., Holst, J. J., Drucker, D. J., Magnan, C., & Burcelin, R. (2011). Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice. Endocrinology, 152(8), 3018-3029. https://doi.org/10.1210/en.2011-0286

Vancouver

Waget A, Cabou C, Masseboeuf M, Cattan P, Armanet M, Karaca M et al. Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice. Endocrinology. 2011 Aug;152(8):3018-3029. https://doi.org/10.1210/en.2011-0286

Author

Waget, Aurélie ; Cabou, Cendrine ; Masseboeuf, Myriam ; Cattan, Pierre ; Armanet, Mattieu ; Karaca, Mélis ; Castel, Julien ; Garret, Celine ; Payros, Gaëlle ; Maida, Adriano ; Sulpice, Thierry ; Holst, Jens Juul ; Drucker, Daniel J ; Magnan, Christophe ; Burcelin, Rémy. / Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice. In: Endocrinology. 2011 ; Vol. 152, No. 8. pp. 3018-3029.

Bibtex

@article{c16b14edeb32429cbfb33badaab2ac91,
title = "Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice",
abstract = "Inhibition of dipeptidyl peptidase-4 (DPP-4) activity improves glucose homeostasis through a mode of action related to the stabilization of the active forms of DPP-4-sensitive hormones such as the incretins that enhance glucose-induced insulin secretion. However, the DPP-4 enzyme is highly expressed on the surface of intestinal epithelial cells; hence, the role of intestinal vs. systemic DPP-4 remains unclear. To analyze mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice, we administered low oral doses of the DPP-4 inhibitor sitagliptin that selectively reduced DPP-4 activity in the intestine. Glp1r(-/-) and Gipr(-/-) mice were studied and glucagon-like peptide (GLP)-1 receptor (GLP-1R) signaling was blocked by an i.v. infusion of the corresponding receptor antagonist exendin (9-39). The role of the dipeptides His-Ala and Tyr-Ala as DPP-4-generated GLP-1 and glucose-dependent insulinotropic peptide (GIP) degradation products was studied in vivo and in vitro on isolated islets. We demonstrate that very low doses of oral sitagliptin improve glucose tolerance and plasma insulin levels with selective reduction of intestinal but not systemic DPP-4 activity. The glucoregulatory action of sitagliptin was associated with increased vagus nerve activity and was diminished in wild-type mice treated with the GLP-1R antagonist exendin (9-39) and in Glp1r(-/-) and Gipr(-/-) mice. Furthermore, the dipeptides liberated from GLP-1 (His-Ala) and GIP (Tyr-Ala) deteriorated glucose tolerance, reduced insulin, and increased portal glucagon levels. The predominant mechanism through which DPP-4 inhibitors regulate glycemia involves local inhibition of intestinal DPP-4 activity, activation of incretin receptors, reduced liberation of bioactive dipeptides, and activation of the gut-to-pancreas neural axis.",
keywords = "Adult, Animals, Blood Glucose, Dipeptides, Dipeptidyl Peptidase 4, Dipeptidyl-Peptidase IV Inhibitors, Glucagon, Glucose Tolerance Test, Humans, Insulin, Male, Mice, Mice, Inbred C57BL, Middle Aged, Pyrazines, Receptors, Gastrointestinal Hormone, Receptors, Glucagon, Triazoles, Vagus Nerve",
author = "Aur{\'e}lie Waget and Cendrine Cabou and Myriam Masseboeuf and Pierre Cattan and Mattieu Armanet and M{\'e}lis Karaca and Julien Castel and Celine Garret and Ga{\"e}lle Payros and Adriano Maida and Thierry Sulpice and Holst, {Jens Juul} and Drucker, {Daniel J} and Christophe Magnan and R{\'e}my Burcelin",
year = "2011",
month = aug,
doi = "10.1210/en.2011-0286",
language = "English",
volume = "152",
pages = "3018--3029",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice

AU - Waget, Aurélie

AU - Cabou, Cendrine

AU - Masseboeuf, Myriam

AU - Cattan, Pierre

AU - Armanet, Mattieu

AU - Karaca, Mélis

AU - Castel, Julien

AU - Garret, Celine

AU - Payros, Gaëlle

AU - Maida, Adriano

AU - Sulpice, Thierry

AU - Holst, Jens Juul

AU - Drucker, Daniel J

AU - Magnan, Christophe

AU - Burcelin, Rémy

PY - 2011/8

Y1 - 2011/8

N2 - Inhibition of dipeptidyl peptidase-4 (DPP-4) activity improves glucose homeostasis through a mode of action related to the stabilization of the active forms of DPP-4-sensitive hormones such as the incretins that enhance glucose-induced insulin secretion. However, the DPP-4 enzyme is highly expressed on the surface of intestinal epithelial cells; hence, the role of intestinal vs. systemic DPP-4 remains unclear. To analyze mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice, we administered low oral doses of the DPP-4 inhibitor sitagliptin that selectively reduced DPP-4 activity in the intestine. Glp1r(-/-) and Gipr(-/-) mice were studied and glucagon-like peptide (GLP)-1 receptor (GLP-1R) signaling was blocked by an i.v. infusion of the corresponding receptor antagonist exendin (9-39). The role of the dipeptides His-Ala and Tyr-Ala as DPP-4-generated GLP-1 and glucose-dependent insulinotropic peptide (GIP) degradation products was studied in vivo and in vitro on isolated islets. We demonstrate that very low doses of oral sitagliptin improve glucose tolerance and plasma insulin levels with selective reduction of intestinal but not systemic DPP-4 activity. The glucoregulatory action of sitagliptin was associated with increased vagus nerve activity and was diminished in wild-type mice treated with the GLP-1R antagonist exendin (9-39) and in Glp1r(-/-) and Gipr(-/-) mice. Furthermore, the dipeptides liberated from GLP-1 (His-Ala) and GIP (Tyr-Ala) deteriorated glucose tolerance, reduced insulin, and increased portal glucagon levels. The predominant mechanism through which DPP-4 inhibitors regulate glycemia involves local inhibition of intestinal DPP-4 activity, activation of incretin receptors, reduced liberation of bioactive dipeptides, and activation of the gut-to-pancreas neural axis.

AB - Inhibition of dipeptidyl peptidase-4 (DPP-4) activity improves glucose homeostasis through a mode of action related to the stabilization of the active forms of DPP-4-sensitive hormones such as the incretins that enhance glucose-induced insulin secretion. However, the DPP-4 enzyme is highly expressed on the surface of intestinal epithelial cells; hence, the role of intestinal vs. systemic DPP-4 remains unclear. To analyze mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice, we administered low oral doses of the DPP-4 inhibitor sitagliptin that selectively reduced DPP-4 activity in the intestine. Glp1r(-/-) and Gipr(-/-) mice were studied and glucagon-like peptide (GLP)-1 receptor (GLP-1R) signaling was blocked by an i.v. infusion of the corresponding receptor antagonist exendin (9-39). The role of the dipeptides His-Ala and Tyr-Ala as DPP-4-generated GLP-1 and glucose-dependent insulinotropic peptide (GIP) degradation products was studied in vivo and in vitro on isolated islets. We demonstrate that very low doses of oral sitagliptin improve glucose tolerance and plasma insulin levels with selective reduction of intestinal but not systemic DPP-4 activity. The glucoregulatory action of sitagliptin was associated with increased vagus nerve activity and was diminished in wild-type mice treated with the GLP-1R antagonist exendin (9-39) and in Glp1r(-/-) and Gipr(-/-) mice. Furthermore, the dipeptides liberated from GLP-1 (His-Ala) and GIP (Tyr-Ala) deteriorated glucose tolerance, reduced insulin, and increased portal glucagon levels. The predominant mechanism through which DPP-4 inhibitors regulate glycemia involves local inhibition of intestinal DPP-4 activity, activation of incretin receptors, reduced liberation of bioactive dipeptides, and activation of the gut-to-pancreas neural axis.

KW - Adult

KW - Animals

KW - Blood Glucose

KW - Dipeptides

KW - Dipeptidyl Peptidase 4

KW - Dipeptidyl-Peptidase IV Inhibitors

KW - Glucagon

KW - Glucose Tolerance Test

KW - Humans

KW - Insulin

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Middle Aged

KW - Pyrazines

KW - Receptors, Gastrointestinal Hormone

KW - Receptors, Glucagon

KW - Triazoles

KW - Vagus Nerve

U2 - 10.1210/en.2011-0286

DO - 10.1210/en.2011-0286

M3 - Journal article

C2 - 21673098

VL - 152

SP - 3018

EP - 3029

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0013-7227

IS - 8

ER -

ID: 38433163