Protocol for qPCR analysis that corrects for cDNA amplification efficiency

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol for qPCR analysis that corrects for cDNA amplification efficiency. / Damgaard, Mads V.; Treebak, Jonas T.

In: STAR Protocols, Vol. 3, No. 3, 101515, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Damgaard, MV & Treebak, JT 2022, 'Protocol for qPCR analysis that corrects for cDNA amplification efficiency', STAR Protocols, vol. 3, no. 3, 101515. https://doi.org/10.1016/j.xpro.2022.101515

APA

Damgaard, M. V., & Treebak, J. T. (2022). Protocol for qPCR analysis that corrects for cDNA amplification efficiency. STAR Protocols, 3(3), [101515]. https://doi.org/10.1016/j.xpro.2022.101515

Vancouver

Damgaard MV, Treebak JT. Protocol for qPCR analysis that corrects for cDNA amplification efficiency. STAR Protocols. 2022;3(3). 101515. https://doi.org/10.1016/j.xpro.2022.101515

Author

Damgaard, Mads V. ; Treebak, Jonas T. / Protocol for qPCR analysis that corrects for cDNA amplification efficiency. In: STAR Protocols. 2022 ; Vol. 3, No. 3.

Bibtex

@article{aa140bb373dd41dfa95ab8aa2e2f3635,
title = "Protocol for qPCR analysis that corrects for cDNA amplification efficiency",
abstract = "This protocol presents a variation on the 2-ΔΔCt technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2-ΔΔCt technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2-ΔΔCt technique, our approach corrects for imperfect cDNA amplification efficiency and allows for the use of multiple housekeeping genes. Collectively, this approach enhances analytical accuracy and thereby reduces the type I and II statistical errors in the generated data.",
keywords = "Gene Expression, Molecular Biology",
author = "Damgaard, {Mads V.} and Treebak, {Jonas T.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
doi = "10.1016/j.xpro.2022.101515",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Protocol for qPCR analysis that corrects for cDNA amplification efficiency

AU - Damgaard, Mads V.

AU - Treebak, Jonas T.

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - This protocol presents a variation on the 2-ΔΔCt technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2-ΔΔCt technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2-ΔΔCt technique, our approach corrects for imperfect cDNA amplification efficiency and allows for the use of multiple housekeeping genes. Collectively, this approach enhances analytical accuracy and thereby reduces the type I and II statistical errors in the generated data.

AB - This protocol presents a variation on the 2-ΔΔCt technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2-ΔΔCt technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2-ΔΔCt technique, our approach corrects for imperfect cDNA amplification efficiency and allows for the use of multiple housekeeping genes. Collectively, this approach enhances analytical accuracy and thereby reduces the type I and II statistical errors in the generated data.

KW - Gene Expression

KW - Molecular Biology

U2 - 10.1016/j.xpro.2022.101515

DO - 10.1016/j.xpro.2022.101515

M3 - Journal article

C2 - 35819886

AN - SCOPUS:85133919103

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 3

M1 - 101515

ER -

ID: 314837572