Time of day determines postexercise metabolism in mouse adipose tissue
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Time of day determines postexercise metabolism in mouse adipose tissue. / Pendergrast, Logan A.; Lundell, Leonidas S.; Ehrlich, Amy M.; Ashcroft, Stephen P.; Schönke, Milena; Basse, Astrid L.; Krook, Anna; Treebak, Jonas T.; Dollet, Lucile; Zierath, Juleen R.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 120, No. 8, e2218510120, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Time of day determines postexercise metabolism in mouse adipose tissue
AU - Pendergrast, Logan A.
AU - Lundell, Leonidas S.
AU - Ehrlich, Amy M.
AU - Ashcroft, Stephen P.
AU - Schönke, Milena
AU - Basse, Astrid L.
AU - Krook, Anna
AU - Treebak, Jonas T.
AU - Dollet, Lucile
AU - Zierath, Juleen R.
N1 - Publisher Copyright: Copyright © 2023 the Author(s).
PY - 2023
Y1 - 2023
N2 - The circadian clock is a cell-autonomous transcription–translation feedback mechanism that anticipates and adapts physiology and behavior to different phases of the day. A variety of factors including hormones, temperature, food-intake, and exercise can act on tissue-specific peripheral clocks to alter the expression of genes that influence metabolism, all in a time-of-day dependent manner. The aim of this study was to elucidate the effects of exercise timing on adipose tissue metabolism. We performed RNA sequencing on inguinal adipose tissue of mice immediately following maximal exercise or sham treatment at the early rest or early active phase. Only during the early active phase did exercise elicit an immediate increase in serum nonesterified fatty acids. Furthermore, early active phase exercise increased expression of markers of thermogenesis and mitochondrial proliferation in inguinal adipose tissue. In vitro, synchronized 3T3-L1 adipocytes showed a timing-dependent difference in Adrb2 expression, as well as a greater lipolytic activity. Thus, the response of adipose tissue to exercise is time-of-day sensitive and may be partly driven by the circadian clock. To determine the influence of feeding state on the time-of-day response to exercise, we replicated the experiment in 10-h-fasted early rest phase mice to mimic the early active phase metabolic status. A 10-h fast led to a similar lipolytic response as observed after active phase exercise but did not replicate the transcriptomic response, suggesting that the observed changes in gene expression are not driven by feeding status. In conclusion, acute exercise elicits timing-specific effects on adipose tissue to maintain metabolic homeostasis.
AB - The circadian clock is a cell-autonomous transcription–translation feedback mechanism that anticipates and adapts physiology and behavior to different phases of the day. A variety of factors including hormones, temperature, food-intake, and exercise can act on tissue-specific peripheral clocks to alter the expression of genes that influence metabolism, all in a time-of-day dependent manner. The aim of this study was to elucidate the effects of exercise timing on adipose tissue metabolism. We performed RNA sequencing on inguinal adipose tissue of mice immediately following maximal exercise or sham treatment at the early rest or early active phase. Only during the early active phase did exercise elicit an immediate increase in serum nonesterified fatty acids. Furthermore, early active phase exercise increased expression of markers of thermogenesis and mitochondrial proliferation in inguinal adipose tissue. In vitro, synchronized 3T3-L1 adipocytes showed a timing-dependent difference in Adrb2 expression, as well as a greater lipolytic activity. Thus, the response of adipose tissue to exercise is time-of-day sensitive and may be partly driven by the circadian clock. To determine the influence of feeding state on the time-of-day response to exercise, we replicated the experiment in 10-h-fasted early rest phase mice to mimic the early active phase metabolic status. A 10-h fast led to a similar lipolytic response as observed after active phase exercise but did not replicate the transcriptomic response, suggesting that the observed changes in gene expression are not driven by feeding status. In conclusion, acute exercise elicits timing-specific effects on adipose tissue to maintain metabolic homeostasis.
KW - adipose tissue
KW - circadian rhythm
KW - exercise
KW - lipolysis
KW - metabolism
U2 - 10.1073/pnas.2218510120
DO - 10.1073/pnas.2218510120
M3 - Journal article
C2 - 36780527
AN - SCOPUS:85147899949
VL - 120
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
M1 - e2218510120
ER -
ID: 337598151