In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells. / Machado, Léo; Esteves de Lima, Joana; Fabre, Odile; Proux, Caroline; Legendre, Rachel; Szegedi, Anikó; Varet, Hugo; Ingerslev, Lars Roed; Barrès, Romain; Relaix, Frédéric; Mourikis, Philippos.

In: Cell Reports, Vol. 21, No. 7, 14.11.2017, p. 1982-1993.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Machado, L, Esteves de Lima, J, Fabre, O, Proux, C, Legendre, R, Szegedi, A, Varet, H, Ingerslev, LR, Barrès, R, Relaix, F & Mourikis, P 2017, 'In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells', Cell Reports, vol. 21, no. 7, pp. 1982-1993. https://doi.org/10.1016/j.celrep.2017.10.080

APA

Machado, L., Esteves de Lima, J., Fabre, O., Proux, C., Legendre, R., Szegedi, A., Varet, H., Ingerslev, L. R., Barrès, R., Relaix, F., & Mourikis, P. (2017). In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells. Cell Reports, 21(7), 1982-1993. https://doi.org/10.1016/j.celrep.2017.10.080

Vancouver

Machado L, Esteves de Lima J, Fabre O, Proux C, Legendre R, Szegedi A et al. In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells. Cell Reports. 2017 Nov 14;21(7):1982-1993. https://doi.org/10.1016/j.celrep.2017.10.080

Author

Machado, Léo ; Esteves de Lima, Joana ; Fabre, Odile ; Proux, Caroline ; Legendre, Rachel ; Szegedi, Anikó ; Varet, Hugo ; Ingerslev, Lars Roed ; Barrès, Romain ; Relaix, Frédéric ; Mourikis, Philippos. / In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells. In: Cell Reports. 2017 ; Vol. 21, No. 7. pp. 1982-1993.

Bibtex

@article{be7b6e7947e34041b19545e303e9968b,
title = "In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells",
abstract = "State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation steps followed by flow cytometry, exposing cells to stress and disrupting their physiological niche. Focusing on adult skeletal muscle stem cells, we have developed a protocol that circumvents the impact of isolation procedures and captures cells in their native quiescent state. We show that current isolation protocols induce major transcriptional changes accompanied by specific histone modifications while having negligible effects on DNA methylation. In addition to proposing a protocol to avoid isolation-induced artifacts, our study reveals previously undetected quiescence and early activation genes of potential biological interest.",
keywords = "Journal Article",
author = "L{\'e}o Machado and {Esteves de Lima}, Joana and Odile Fabre and Caroline Proux and Rachel Legendre and Anik{\'o} Szegedi and Hugo Varet and Ingerslev, {Lars Roed} and Romain Barr{\`e}s and Fr{\'e}d{\'e}ric Relaix and Philippos Mourikis",
note = "Copyright {\textcopyright} 2017 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2017",
month = nov,
day = "14",
doi = "10.1016/j.celrep.2017.10.080",
language = "English",
volume = "21",
pages = "1982--1993",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",

}

RIS

TY - JOUR

T1 - In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells

AU - Machado, Léo

AU - Esteves de Lima, Joana

AU - Fabre, Odile

AU - Proux, Caroline

AU - Legendre, Rachel

AU - Szegedi, Anikó

AU - Varet, Hugo

AU - Ingerslev, Lars Roed

AU - Barrès, Romain

AU - Relaix, Frédéric

AU - Mourikis, Philippos

N1 - Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2017/11/14

Y1 - 2017/11/14

N2 - State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation steps followed by flow cytometry, exposing cells to stress and disrupting their physiological niche. Focusing on adult skeletal muscle stem cells, we have developed a protocol that circumvents the impact of isolation procedures and captures cells in their native quiescent state. We show that current isolation protocols induce major transcriptional changes accompanied by specific histone modifications while having negligible effects on DNA methylation. In addition to proposing a protocol to avoid isolation-induced artifacts, our study reveals previously undetected quiescence and early activation genes of potential biological interest.

AB - State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation steps followed by flow cytometry, exposing cells to stress and disrupting their physiological niche. Focusing on adult skeletal muscle stem cells, we have developed a protocol that circumvents the impact of isolation procedures and captures cells in their native quiescent state. We show that current isolation protocols induce major transcriptional changes accompanied by specific histone modifications while having negligible effects on DNA methylation. In addition to proposing a protocol to avoid isolation-induced artifacts, our study reveals previously undetected quiescence and early activation genes of potential biological interest.

KW - Journal Article

U2 - 10.1016/j.celrep.2017.10.080

DO - 10.1016/j.celrep.2017.10.080

M3 - Journal article

C2 - 29141227

VL - 21

SP - 1982

EP - 1993

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 7

ER -

ID: 189863992