Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine. / Orskov, C; Buhl, T; Rabenhøj, L; Kofod, Hans; Holst, J J.

In: F E B S Letters, Vol. 247, No. 2, 24.04.1989, p. 193-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Orskov, C, Buhl, T, Rabenhøj, L, Kofod, H & Holst, JJ 1989, 'Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine', F E B S Letters, vol. 247, no. 2, pp. 193-6.

APA

Orskov, C., Buhl, T., Rabenhøj, L., Kofod, H., & Holst, J. J. (1989). Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine. F E B S Letters, 247(2), 193-6.

Vancouver

Orskov C, Buhl T, Rabenhøj L, Kofod H, Holst JJ. Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine. F E B S Letters. 1989 Apr 24;247(2):193-6.

Author

Orskov, C ; Buhl, T ; Rabenhøj, L ; Kofod, Hans ; Holst, J J. / Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine. In: F E B S Letters. 1989 ; Vol. 247, No. 2. pp. 193-6.

Bibtex

@article{2c54e57c8f1c4d4d888a73acb6a8fa63,
title = "Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine",
abstract = "We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.",
keywords = "Amino Acid Sequence, Animals, Carboxypeptidase B, Carboxypeptidases, Chromatography, Gel, Gastrointestinal Hormones, Glucagon-Like Peptide 2, Glucagon-Like Peptides, Humans, Intestine, Small, Molecular Sequence Data, Peptide Fragments, Peptides, Protein Precursors, Protein Processing, Post-Translational, Radioimmunoassay, Swine",
author = "C Orskov and T Buhl and L Rabenh{\o}j and Hans Kofod and Holst, {J J}",
year = "1989",
month = apr,
day = "24",
language = "English",
volume = "247",
pages = "193--6",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Carboxypeptidase-B-like processing of the C-terminus of glucagon-like peptide-2 in pig and human small intestine

AU - Orskov, C

AU - Buhl, T

AU - Rabenhøj, L

AU - Kofod, Hans

AU - Holst, J J

PY - 1989/4/24

Y1 - 1989/4/24

N2 - We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.

AB - We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151-158, PG 151-160 and PG 126-159 (glucagon-like peptide-2 (GLP-2] in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126-159) and for PG 151-158, whereas the assay for PG 151-160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.

KW - Amino Acid Sequence

KW - Animals

KW - Carboxypeptidase B

KW - Carboxypeptidases

KW - Chromatography, Gel

KW - Gastrointestinal Hormones

KW - Glucagon-Like Peptide 2

KW - Glucagon-Like Peptides

KW - Humans

KW - Intestine, Small

KW - Molecular Sequence Data

KW - Peptide Fragments

KW - Peptides

KW - Protein Precursors

KW - Protein Processing, Post-Translational

KW - Radioimmunoassay

KW - Swine

M3 - Journal article

C2 - 2714431

VL - 247

SP - 193

EP - 196

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 2

ER -

ID: 45575003