Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum

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Standard

Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum. / Messell, T; Harling, H; Poulsen, Steen Seier; Bersani, M; Holst, J J.

In: Regulatory Peptides, Vol. 38, No. 3, 09.04.1992, p. 179-98.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Messell, T, Harling, H, Poulsen, SS, Bersani, M & Holst, JJ 1992, 'Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum', Regulatory Peptides, vol. 38, no. 3, pp. 179-98.

APA

Messell, T., Harling, H., Poulsen, S. S., Bersani, M., & Holst, J. J. (1992). Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum. Regulatory Peptides, 38(3), 179-98.

Vancouver

Messell T, Harling H, Poulsen SS, Bersani M, Holst JJ. Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum. Regulatory Peptides. 1992 Apr 9;38(3):179-98.

Author

Messell, T ; Harling, H ; Poulsen, Steen Seier ; Bersani, M ; Holst, J J. / Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum. In: Regulatory Peptides. 1992 ; Vol. 38, No. 3. pp. 179-98.

Bibtex

@article{a5fa4eebb2f146cbabefd57257336ab6,
title = "Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum",
abstract = "By immunohistochemistry galanin-like immunoreactivity and vasoactive intestinal polypeptide (VIP)-like immunoreactivity were found in nerve cell bodies mostly in the submucous plexus and in nerve fibres in the mucosa, submucosa and muscularis including the myenteric plexus of the porcine ileum and were found to co-exist in most of these structures. Using isolated, perfused porcine ileum we studied the release of galanin and VIP in response to electrical stimulation of the mixed periarterial nerves or to intraarterial infusions of different neuroactive agents. Nerve stimulation (4-10 Hz) inhibited the basal release of galanin and VIP from the ileum (to 69 +/- 6 and 62 +/- 6% of basal release). After infusion of the alpha-adrenergic blocker, phentolamine, (10(-6) M) electrical stimulation increased the release of both galanin and VIP (to 140 +/- 12 and 133 +/- 13% of basal output). This increase was abolished by atropine (10(-6) M) and by hexamethonium (3.10(-5) M). Infusion of norepinephrine (10(-6) M) inhibited, whereas acetylcholine (10(-6) M) stimulated the release of both peptides. The effect of the latter was abolished by atropine. The inhibitory effect of nerve stimulation was not influenced by atropine. Our results suggest that the galanin- and VIP-producing intrinsic neurons receive inhibitory signals by noradrenergic nerve fibers and stimulatory signals mediated by cholinergic nerves, possibly via a cholinergic interneuron.",
keywords = "Acetylcholine, Animals, Atropine, Electric Stimulation, Galanin, Ileum, Intestinal Mucosa, Microscopy, Fluorescence, Myenteric Plexus, Nerve Fibers, Neuropeptides, Norepinephrine, Peptides, Phentolamine, Radioimmunoassay, Vasoactive Intestinal Peptide",
author = "T Messell and H Harling and Poulsen, {Steen Seier} and M Bersani and Holst, {J J}",
year = "1992",
month = apr,
day = "9",
language = "English",
volume = "38",
pages = "179--98",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused, porcine ileum

AU - Messell, T

AU - Harling, H

AU - Poulsen, Steen Seier

AU - Bersani, M

AU - Holst, J J

PY - 1992/4/9

Y1 - 1992/4/9

N2 - By immunohistochemistry galanin-like immunoreactivity and vasoactive intestinal polypeptide (VIP)-like immunoreactivity were found in nerve cell bodies mostly in the submucous plexus and in nerve fibres in the mucosa, submucosa and muscularis including the myenteric plexus of the porcine ileum and were found to co-exist in most of these structures. Using isolated, perfused porcine ileum we studied the release of galanin and VIP in response to electrical stimulation of the mixed periarterial nerves or to intraarterial infusions of different neuroactive agents. Nerve stimulation (4-10 Hz) inhibited the basal release of galanin and VIP from the ileum (to 69 +/- 6 and 62 +/- 6% of basal release). After infusion of the alpha-adrenergic blocker, phentolamine, (10(-6) M) electrical stimulation increased the release of both galanin and VIP (to 140 +/- 12 and 133 +/- 13% of basal output). This increase was abolished by atropine (10(-6) M) and by hexamethonium (3.10(-5) M). Infusion of norepinephrine (10(-6) M) inhibited, whereas acetylcholine (10(-6) M) stimulated the release of both peptides. The effect of the latter was abolished by atropine. The inhibitory effect of nerve stimulation was not influenced by atropine. Our results suggest that the galanin- and VIP-producing intrinsic neurons receive inhibitory signals by noradrenergic nerve fibers and stimulatory signals mediated by cholinergic nerves, possibly via a cholinergic interneuron.

AB - By immunohistochemistry galanin-like immunoreactivity and vasoactive intestinal polypeptide (VIP)-like immunoreactivity were found in nerve cell bodies mostly in the submucous plexus and in nerve fibres in the mucosa, submucosa and muscularis including the myenteric plexus of the porcine ileum and were found to co-exist in most of these structures. Using isolated, perfused porcine ileum we studied the release of galanin and VIP in response to electrical stimulation of the mixed periarterial nerves or to intraarterial infusions of different neuroactive agents. Nerve stimulation (4-10 Hz) inhibited the basal release of galanin and VIP from the ileum (to 69 +/- 6 and 62 +/- 6% of basal release). After infusion of the alpha-adrenergic blocker, phentolamine, (10(-6) M) electrical stimulation increased the release of both galanin and VIP (to 140 +/- 12 and 133 +/- 13% of basal output). This increase was abolished by atropine (10(-6) M) and by hexamethonium (3.10(-5) M). Infusion of norepinephrine (10(-6) M) inhibited, whereas acetylcholine (10(-6) M) stimulated the release of both peptides. The effect of the latter was abolished by atropine. The inhibitory effect of nerve stimulation was not influenced by atropine. Our results suggest that the galanin- and VIP-producing intrinsic neurons receive inhibitory signals by noradrenergic nerve fibers and stimulatory signals mediated by cholinergic nerves, possibly via a cholinergic interneuron.

KW - Acetylcholine

KW - Animals

KW - Atropine

KW - Electric Stimulation

KW - Galanin

KW - Ileum

KW - Intestinal Mucosa

KW - Microscopy, Fluorescence

KW - Myenteric Plexus

KW - Nerve Fibers

KW - Neuropeptides

KW - Norepinephrine

KW - Peptides

KW - Phentolamine

KW - Radioimmunoassay

KW - Vasoactive Intestinal Peptide

M3 - Journal article

C2 - 1375382

VL - 38

SP - 179

EP - 198

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3

ER -

ID: 47487648