Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine
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Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine. / Buhl, T; Thim, L; Kofod, Hans; Orskov, C; Harling, H; Holst, J J.
In: Journal of Biological Chemistry, Vol. 263, No. 18, 25.06.1988, p. 8621-4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Naturally occurring products of proglucagon 111-160 in the porcine and human small intestine
AU - Buhl, T
AU - Thim, L
AU - Kofod, Hans
AU - Orskov, C
AU - Harling, H
AU - Holst, J J
PY - 1988/6/25
Y1 - 1988/6/25
N2 - Recent studies have revealed that the glucagon gene is expressed in the mammalian intestine. Here it codes for "glicentin" (proglucagon 1-69) and a glucagon-like peptide, proglucagon 78-107, recently isolated from porcine intestine. We studied the fate of the remaining COOH-terminal part of proglucagon (proglucagon 111-160) using radioimmunoassays against proglucagon 111-123 and 126-160. Two peptides were isolated from acid ethanol extracts of porcine ileal mucosa and sequenced: one corresponding to proglucagon 126-158 and one probably corresponding to proglucagon 111-158. By comparing human and porcine proglucagon sequences, Ala117 is replaced by Thr, and Ile138, Ala144, Ile152 and Gln153 are replaced by Val, Thr, Leu, and His. By gel filtration and radioimmunoassay of intestinal extracts it was established that a large part of porcine and virtually all of human proglucagon are processed to release proglucagon 111-123 (designated spacer peptide 2), which, like proglucagon 126-158 must be considered a potential hormonal entity. By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide.
AB - Recent studies have revealed that the glucagon gene is expressed in the mammalian intestine. Here it codes for "glicentin" (proglucagon 1-69) and a glucagon-like peptide, proglucagon 78-107, recently isolated from porcine intestine. We studied the fate of the remaining COOH-terminal part of proglucagon (proglucagon 111-160) using radioimmunoassays against proglucagon 111-123 and 126-160. Two peptides were isolated from acid ethanol extracts of porcine ileal mucosa and sequenced: one corresponding to proglucagon 126-158 and one probably corresponding to proglucagon 111-158. By comparing human and porcine proglucagon sequences, Ala117 is replaced by Thr, and Ile138, Ala144, Ile152 and Gln153 are replaced by Val, Thr, Leu, and His. By gel filtration and radioimmunoassay of intestinal extracts it was established that a large part of porcine and virtually all of human proglucagon are processed to release proglucagon 111-123 (designated spacer peptide 2), which, like proglucagon 126-158 must be considered a potential hormonal entity. By isocratic high pressure liquid chromatography human spacer peptide 2 was indistinguishable from synthetic proglucagon 111-122 amide, suggesting that this is the structure of the naturally occurring human peptide.
KW - Amino Acid Sequence
KW - Animals
KW - Glucagon
KW - Humans
KW - Ileum
KW - Intestinal Mucosa
KW - Molecular Sequence Data
KW - Peptide Fragments
KW - Protein Precursors
KW - Radioimmunoassay
KW - Species Specificity
KW - Swine
M3 - Journal article
C2 - 3379036
VL - 263
SP - 8621
EP - 8624
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 18
ER -
ID: 45575151