Tachykinins in the porcine pancreas: potent exocrine and endocrine effects via NK-1 receptors
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Tachykinins in the porcine pancreas : potent exocrine and endocrine effects via NK-1 receptors. / Schmidt, P T; Tornøe, K; Poulsen, Steen Seier; Rasmussen, T N; Holst, J J.
In: Pancreas, Vol. 20, No. 3, 04.2000, p. 241-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tachykinins in the porcine pancreas
T2 - potent exocrine and endocrine effects via NK-1 receptors
AU - Schmidt, P T
AU - Tornøe, K
AU - Poulsen, Steen Seier
AU - Rasmussen, T N
AU - Holst, J J
PY - 2000/4
Y1 - 2000/4
N2 - The localization, release, and effects of substance P and neurokinin A were studied in the porcine pancreas and the localization of substance P immunoreactive nerve fibers was examined by immunohistochemistry. The effects of electrical vagus stimulation and capsaicin infusion on tachykinin release and the effects of substance P and neurokinin A infusion on insulin, glucagon, somatostatin, and exocrine secretion were studied using the isolated perfused porcine pancreas with intact vagal innervation. NK-1 and NK-2 receptor antagonists were used to investigate receptor involvement. Substance P immunoreactive nerve fibers were localized to islets of Langerhans, acini, ducts, and blood vessels. Vagus stimulation had no effect on substance P and neurokinin A release, whereas capsaicin infusion stimulated release of both. Substance P and neurokinin A infusion increased release of insulin, glucagon, and exocrine secretion, whereas somatostatin secretion was unaffected. The effect of substance P on insulin, glucagon, and exocrine secretion was blocked by the NK-1 receptor antagonist. The effect of electrical stimulation of vagus nerves on insulin and exocrine secretion was not influenced by tachykinin receptor antagonists. We conclude that tachykinins stimulate both endocrine and exocrine pancreatic functions through NK-1 receptors. Tachykinins are not involved in vagal regulation of pancreatic secretion in pigs but could constitute part of an alternative stimulatory system.
AB - The localization, release, and effects of substance P and neurokinin A were studied in the porcine pancreas and the localization of substance P immunoreactive nerve fibers was examined by immunohistochemistry. The effects of electrical vagus stimulation and capsaicin infusion on tachykinin release and the effects of substance P and neurokinin A infusion on insulin, glucagon, somatostatin, and exocrine secretion were studied using the isolated perfused porcine pancreas with intact vagal innervation. NK-1 and NK-2 receptor antagonists were used to investigate receptor involvement. Substance P immunoreactive nerve fibers were localized to islets of Langerhans, acini, ducts, and blood vessels. Vagus stimulation had no effect on substance P and neurokinin A release, whereas capsaicin infusion stimulated release of both. Substance P and neurokinin A infusion increased release of insulin, glucagon, and exocrine secretion, whereas somatostatin secretion was unaffected. The effect of substance P on insulin, glucagon, and exocrine secretion was blocked by the NK-1 receptor antagonist. The effect of electrical stimulation of vagus nerves on insulin and exocrine secretion was not influenced by tachykinin receptor antagonists. We conclude that tachykinins stimulate both endocrine and exocrine pancreatic functions through NK-1 receptors. Tachykinins are not involved in vagal regulation of pancreatic secretion in pigs but could constitute part of an alternative stimulatory system.
KW - Animals
KW - Capsaicin
KW - Electric Stimulation
KW - Glucagon
KW - Immunohistochemistry
KW - Insulin
KW - Islets of Langerhans
KW - Nerve Fibers
KW - Neurokinin A
KW - Pancreas
KW - Receptors, Neurokinin-1
KW - Somatostatin
KW - Substance P
KW - Swine
KW - Tachykinins
KW - Vagus Nerve
M3 - Journal article
C2 - 10766449
VL - 20
SP - 241
EP - 247
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 3
ER -
ID: 47486461