Oligogenic Architecture of Rare Noncoding Variants Distinguishes 4 Congenital Heart Disease Phenotypes
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Oligogenic Architecture of Rare Noncoding Variants Distinguishes 4 Congenital Heart Disease Phenotypes. / Yu, Mengyao; Aguirre, Matthew; Jia, Meiwen; Gjoni, Ketrin; Cordova-Palomera, Aldo; Munger, Chad; Amgalan, Dulguun; Rosa Ma, X.; Pereira, Alexandre; Tcheandjieu, Catherine; Seidman, Christine; Seidman, Jonathan; Tristani-Firouzi, Martin; Chung, Wendy; Goldmuntz, Elizabeth; Srivastava, Deepak; Loos, Ruth J.F.; Chami, Nathalie; Cordell, Heather; Dreßen, Martina; Mueller-Myhsok, Bertram; Lahm, Harald; Krane, Markus; Pollard, Katherine S.; Engreitz, Jesse M.; Gagliano Taliun, Sarah A.; Gelb, Bruce D.; Priest, James R.
In: Circulation: Genomic and Precision Medicine, Vol. 16, No. 3, 2023, p. 258-266.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Oligogenic Architecture of Rare Noncoding Variants Distinguishes 4 Congenital Heart Disease Phenotypes
AU - Yu, Mengyao
AU - Aguirre, Matthew
AU - Jia, Meiwen
AU - Gjoni, Ketrin
AU - Cordova-Palomera, Aldo
AU - Munger, Chad
AU - Amgalan, Dulguun
AU - Rosa Ma, X.
AU - Pereira, Alexandre
AU - Tcheandjieu, Catherine
AU - Seidman, Christine
AU - Seidman, Jonathan
AU - Tristani-Firouzi, Martin
AU - Chung, Wendy
AU - Goldmuntz, Elizabeth
AU - Srivastava, Deepak
AU - Loos, Ruth J.F.
AU - Chami, Nathalie
AU - Cordell, Heather
AU - Dreßen, Martina
AU - Mueller-Myhsok, Bertram
AU - Lahm, Harald
AU - Krane, Markus
AU - Pollard, Katherine S.
AU - Engreitz, Jesse M.
AU - Gagliano Taliun, Sarah A.
AU - Gelb, Bruce D.
AU - Priest, James R.
N1 - Publisher Copyright: © 2023 Lippincott Williams and Wilkins. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Background: Congenital heart disease (CHD) is highly heritable, but the power to identify inherited risk has been limited to analyses of common variants in small cohorts. Methods: We performed reimputation of 4 CHD cohorts (n=55 342) to the TOPMed reference panel (freeze 5), permitting meta-analysis of 14 784 017 variants including 6 035 962 rare variants of high imputation quality as validated by whole genome sequencing. Results: Meta-analysis identified 16 novel loci, including 12 rare variants, which displayed moderate or large effect sizes (median odds ratio, 3.02) for 4 separate CHD categories. Analyses of chromatin structure link 13 of the genome-wide significant loci to key genes in cardiac development; rs373447426 (minor allele frequency, 0.003 [odds ratio, 3.37 for Conotruncal heart disease]; P=1.49×10-8) is predicted to disrupt chromatin structure for 2 nearby genes BDH1 and DLG1 involved in Conotruncal development. A lead variant rs189203952 (minor allele frequency, 0.01 [odds ratio, 2.4 for left ventricular outflow tract obstruction]; P=1.46×10-8) is predicted to disrupt the binding sites of 4 transcription factors known to participate in cardiac development in the promoter of SPAG9. A tissue-specific model of chromatin conformation suggests that common variant rs78256848 (minor allele frequency, 0.11 [odds ratio, 1.4 for Conotruncal heart disease]; P=2.6×10-8) physically interacts with NCAM1 (PFDR=1.86×10-27), a neural adhesion molecule acting in cardiac development. Importantly, while each individual malformation displayed substantial heritability (observed h2 ranging from 0.26 for complex malformations to 0.37 for left ventricular outflow tract obstructive disease) the risk for different CHD malformations appeared to be separate, without genetic correlation measured by linkage disequilibrium score regression or regional colocalization. Conclusions: We describe a set of rare noncoding variants conferring significant risk for individual heart malformations which are linked to genes governing cardiac development. These results illustrate that the oligogenic basis of CHD and significant heritability may be linked to rare variants outside protein-coding regions conferring substantial risk for individual categories of cardiac malformation.
AB - Background: Congenital heart disease (CHD) is highly heritable, but the power to identify inherited risk has been limited to analyses of common variants in small cohorts. Methods: We performed reimputation of 4 CHD cohorts (n=55 342) to the TOPMed reference panel (freeze 5), permitting meta-analysis of 14 784 017 variants including 6 035 962 rare variants of high imputation quality as validated by whole genome sequencing. Results: Meta-analysis identified 16 novel loci, including 12 rare variants, which displayed moderate or large effect sizes (median odds ratio, 3.02) for 4 separate CHD categories. Analyses of chromatin structure link 13 of the genome-wide significant loci to key genes in cardiac development; rs373447426 (minor allele frequency, 0.003 [odds ratio, 3.37 for Conotruncal heart disease]; P=1.49×10-8) is predicted to disrupt chromatin structure for 2 nearby genes BDH1 and DLG1 involved in Conotruncal development. A lead variant rs189203952 (minor allele frequency, 0.01 [odds ratio, 2.4 for left ventricular outflow tract obstruction]; P=1.46×10-8) is predicted to disrupt the binding sites of 4 transcription factors known to participate in cardiac development in the promoter of SPAG9. A tissue-specific model of chromatin conformation suggests that common variant rs78256848 (minor allele frequency, 0.11 [odds ratio, 1.4 for Conotruncal heart disease]; P=2.6×10-8) physically interacts with NCAM1 (PFDR=1.86×10-27), a neural adhesion molecule acting in cardiac development. Importantly, while each individual malformation displayed substantial heritability (observed h2 ranging from 0.26 for complex malformations to 0.37 for left ventricular outflow tract obstructive disease) the risk for different CHD malformations appeared to be separate, without genetic correlation measured by linkage disequilibrium score regression or regional colocalization. Conclusions: We describe a set of rare noncoding variants conferring significant risk for individual heart malformations which are linked to genes governing cardiac development. These results illustrate that the oligogenic basis of CHD and significant heritability may be linked to rare variants outside protein-coding regions conferring substantial risk for individual categories of cardiac malformation.
KW - alleles
KW - chromatin
KW - live birth
KW - phenotype
KW - prevalence
U2 - 10.1161/CIRCGEN.122.003968
DO - 10.1161/CIRCGEN.122.003968
M3 - Journal article
C2 - 37026454
AN - SCOPUS:85163514921
VL - 16
SP - 258
EP - 266
JO - Circulation. Genomic and precision medicine
JF - Circulation. Genomic and precision medicine
SN - 2574-8300
IS - 3
ER -
ID: 361159776