Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots
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Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots. / Palani, Nagendra P.; Horvath, Carla; Timshel, Pascal N.; Folkertsma, Pytrik; Grønning, Alexander G.B.; Henriksen, Tora I.; Peijs, Lone; Jensen, Verena H.; Sun, Wenfei; Jespersen, Naja Z.; Wolfrum, Christian; Pers, Tune H.; Nielsen, Søren; Scheele, Camilla.
In: Nature Metabolism, Vol. 5, No. 6, 2023, p. 996-1013.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots
AU - Palani, Nagendra P.
AU - Horvath, Carla
AU - Timshel, Pascal N.
AU - Folkertsma, Pytrik
AU - Grønning, Alexander G.B.
AU - Henriksen, Tora I.
AU - Peijs, Lone
AU - Jensen, Verena H.
AU - Sun, Wenfei
AU - Jespersen, Naja Z.
AU - Wolfrum, Christian
AU - Pers, Tune H.
AU - Nielsen, Søren
AU - Scheele, Camilla
N1 - Publisher Copyright: © 2023, The Author(s).
PY - 2023
Y1 - 2023
N2 - Adipocyte function is a major determinant of metabolic disease, warranting investigations of regulating mechanisms. We show at single-cell resolution that progenitor cells from four human brown and white adipose depots separate into two main cell fates, an adipogenic and a structural branch, developing from a common progenitor. The adipogenic gene signature contains mitochondrial activity genes, and associates with genome-wide association study traits for fat distribution. Based on an extracellular matrix and developmental gene signature, we name the structural branch of cells structural Wnt-regulated adipose tissue-resident (SWAT) cells. When stripped from adipogenic cells, SWAT cells display a multipotent phenotype by reverting towards progenitor state or differentiating into new adipogenic cells, dependent on media. Label transfer algorithms recapitulate the cell types in human adipose tissue datasets. In conclusion, we provide a differentiation map of human adipocytes and define the multipotent SWAT cell, providing a new perspective on adipose tissue regulation.
AB - Adipocyte function is a major determinant of metabolic disease, warranting investigations of regulating mechanisms. We show at single-cell resolution that progenitor cells from four human brown and white adipose depots separate into two main cell fates, an adipogenic and a structural branch, developing from a common progenitor. The adipogenic gene signature contains mitochondrial activity genes, and associates with genome-wide association study traits for fat distribution. Based on an extracellular matrix and developmental gene signature, we name the structural branch of cells structural Wnt-regulated adipose tissue-resident (SWAT) cells. When stripped from adipogenic cells, SWAT cells display a multipotent phenotype by reverting towards progenitor state or differentiating into new adipogenic cells, dependent on media. Label transfer algorithms recapitulate the cell types in human adipose tissue datasets. In conclusion, we provide a differentiation map of human adipocytes and define the multipotent SWAT cell, providing a new perspective on adipose tissue regulation.
U2 - 10.1038/s42255-023-00820-z
DO - 10.1038/s42255-023-00820-z
M3 - Journal article
C2 - 37337126
AN - SCOPUS:85161976894
VL - 5
SP - 996
EP - 1013
JO - Nature Metabolism
JF - Nature Metabolism
SN - 2522-5812
IS - 6
ER -
ID: 361390242