Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice. / Fischer, Alexander W.; Behrens, Janina; Sass, Frederike; Schlein, Christian; Heine, Markus; Pertzborn, Paul; Scheja, Ludger; Heeren, Joerg.

In: Journal of Lipid Research, Vol. 61, No. 11, 2020, p. 1377-1389.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fischer, AW, Behrens, J, Sass, F, Schlein, C, Heine, M, Pertzborn, P, Scheja, L & Heeren, J 2020, 'Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice', Journal of Lipid Research, vol. 61, no. 11, pp. 1377-1389. https://doi.org/10.1194/jlr.RA119000455

APA

Fischer, A. W., Behrens, J., Sass, F., Schlein, C., Heine, M., Pertzborn, P., Scheja, L., & Heeren, J. (2020). Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice. Journal of Lipid Research, 61(11), 1377-1389. https://doi.org/10.1194/jlr.RA119000455

Vancouver

Fischer AW, Behrens J, Sass F, Schlein C, Heine M, Pertzborn P et al. Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice. Journal of Lipid Research. 2020;61(11):1377-1389. https://doi.org/10.1194/jlr.RA119000455

Author

Fischer, Alexander W. ; Behrens, Janina ; Sass, Frederike ; Schlein, Christian ; Heine, Markus ; Pertzborn, Paul ; Scheja, Ludger ; Heeren, Joerg. / Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice. In: Journal of Lipid Research. 2020 ; Vol. 61, No. 11. pp. 1377-1389.

Bibtex

@article{5bb01d5d72694a43bdefffebd0247a12,
title = "Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice",
abstract = "Adaptive thermogenesis is highly dependent on uncoupling protein 1 (UCP1), a protein expressed by thermogenic adipocytes present in brown adipose tissue (BAT) and white adipose tissue (WAT). Thermogenic capacity of human and mouse BAT can be measured by positron emission tomography-computed tomography quantifying the uptake of F-18-fluodeoxyglucose or lipid tracers. BAT activation is typically studied in response to cold exposure or treatment with beta-3-adrenergic receptor agonists such as CL316,243 (CL). Currently, it is unknown whether cold-stimulated uptake of glucose or lipid tracers is a good surrogate marker of UCP1-mediated thermogenesis. In metabolic studies using radiolabeled tracers, we found that glucose uptake is increased in mildly cold-activated BAT of Ucp1(-/-) versus WT mice kept at subthermoneutral temperature. Conversely, lower glucose disposal was detected after full thermogenic activation achieved by sustained cold exposure or CL treatment. In contrast, uptake of lipoprotein-derived fatty acids into chronically activated thermogenic adipose tissues was substantially increased in UCP1-deficient mice. This effect is linked to higher sympathetic tone in adipose tissues of Ucp1(-/-) mice, as indicated by elevated levels of thermogenic genes in BAT and WAT. Thus, glucose and lipoprotein handling does not necessarily reflect UCP1-dependent thermogenic activity, but especially lipid uptake rather mirrors sympathetic activation of adipose tissues.",
keywords = "adipocytes, lipase, lipoprotein, lipid metabolism, hormone-sensitive, lipid transport, lipolysis and fatty acid metabolism, lipoprotein metabolism, triglycerides, MUSCLE-BASED THERMOGENESIS, DIET-INDUCED THERMOGENESIS, FATTY-ACID TRANSPORT, ENERGY-EXPENDITURE, NONSHIVERING THERMOGENESIS, OXIDATIVE-METABOLISM, COLD-EXPOSURE, UCP1, WHITE, FGF21",
author = "Fischer, {Alexander W.} and Janina Behrens and Frederike Sass and Christian Schlein and Markus Heine and Paul Pertzborn and Ludger Scheja and Joerg Heeren",
year = "2020",
doi = "10.1194/jlr.RA119000455",
language = "English",
volume = "61",
pages = "1377--1389",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "11",

}

RIS

TY - JOUR

T1 - Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice

AU - Fischer, Alexander W.

AU - Behrens, Janina

AU - Sass, Frederike

AU - Schlein, Christian

AU - Heine, Markus

AU - Pertzborn, Paul

AU - Scheja, Ludger

AU - Heeren, Joerg

PY - 2020

Y1 - 2020

N2 - Adaptive thermogenesis is highly dependent on uncoupling protein 1 (UCP1), a protein expressed by thermogenic adipocytes present in brown adipose tissue (BAT) and white adipose tissue (WAT). Thermogenic capacity of human and mouse BAT can be measured by positron emission tomography-computed tomography quantifying the uptake of F-18-fluodeoxyglucose or lipid tracers. BAT activation is typically studied in response to cold exposure or treatment with beta-3-adrenergic receptor agonists such as CL316,243 (CL). Currently, it is unknown whether cold-stimulated uptake of glucose or lipid tracers is a good surrogate marker of UCP1-mediated thermogenesis. In metabolic studies using radiolabeled tracers, we found that glucose uptake is increased in mildly cold-activated BAT of Ucp1(-/-) versus WT mice kept at subthermoneutral temperature. Conversely, lower glucose disposal was detected after full thermogenic activation achieved by sustained cold exposure or CL treatment. In contrast, uptake of lipoprotein-derived fatty acids into chronically activated thermogenic adipose tissues was substantially increased in UCP1-deficient mice. This effect is linked to higher sympathetic tone in adipose tissues of Ucp1(-/-) mice, as indicated by elevated levels of thermogenic genes in BAT and WAT. Thus, glucose and lipoprotein handling does not necessarily reflect UCP1-dependent thermogenic activity, but especially lipid uptake rather mirrors sympathetic activation of adipose tissues.

AB - Adaptive thermogenesis is highly dependent on uncoupling protein 1 (UCP1), a protein expressed by thermogenic adipocytes present in brown adipose tissue (BAT) and white adipose tissue (WAT). Thermogenic capacity of human and mouse BAT can be measured by positron emission tomography-computed tomography quantifying the uptake of F-18-fluodeoxyglucose or lipid tracers. BAT activation is typically studied in response to cold exposure or treatment with beta-3-adrenergic receptor agonists such as CL316,243 (CL). Currently, it is unknown whether cold-stimulated uptake of glucose or lipid tracers is a good surrogate marker of UCP1-mediated thermogenesis. In metabolic studies using radiolabeled tracers, we found that glucose uptake is increased in mildly cold-activated BAT of Ucp1(-/-) versus WT mice kept at subthermoneutral temperature. Conversely, lower glucose disposal was detected after full thermogenic activation achieved by sustained cold exposure or CL treatment. In contrast, uptake of lipoprotein-derived fatty acids into chronically activated thermogenic adipose tissues was substantially increased in UCP1-deficient mice. This effect is linked to higher sympathetic tone in adipose tissues of Ucp1(-/-) mice, as indicated by elevated levels of thermogenic genes in BAT and WAT. Thus, glucose and lipoprotein handling does not necessarily reflect UCP1-dependent thermogenic activity, but especially lipid uptake rather mirrors sympathetic activation of adipose tissues.

KW - adipocytes

KW - lipase

KW - lipoprotein

KW - lipid metabolism

KW - hormone-sensitive

KW - lipid transport

KW - lipolysis and fatty acid metabolism

KW - lipoprotein metabolism

KW - triglycerides

KW - MUSCLE-BASED THERMOGENESIS

KW - DIET-INDUCED THERMOGENESIS

KW - FATTY-ACID TRANSPORT

KW - ENERGY-EXPENDITURE

KW - NONSHIVERING THERMOGENESIS

KW - OXIDATIVE-METABOLISM

KW - COLD-EXPOSURE

KW - UCP1

KW - WHITE

KW - FGF21

U2 - 10.1194/jlr.RA119000455

DO - 10.1194/jlr.RA119000455

M3 - Journal article

C2 - 32769145

VL - 61

SP - 1377

EP - 1389

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 11

ER -

ID: 251945394