Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice
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Adaptive thermogenesis is highly dependent on uncoupling protein 1 (UCP1), a protein expressed by thermogenic adipocytes present in brown adipose tissue (BAT) and white adipose tissue (WAT). Thermogenic capacity of human and mouse BAT can be measured by positron emission tomography-computed tomography quantifying the uptake of F-18-fluodeoxyglucose or lipid tracers. BAT activation is typically studied in response to cold exposure or treatment with beta-3-adrenergic receptor agonists such as CL316,243 (CL). Currently, it is unknown whether cold-stimulated uptake of glucose or lipid tracers is a good surrogate marker of UCP1-mediated thermogenesis. In metabolic studies using radiolabeled tracers, we found that glucose uptake is increased in mildly cold-activated BAT of Ucp1(-/-) versus WT mice kept at subthermoneutral temperature. Conversely, lower glucose disposal was detected after full thermogenic activation achieved by sustained cold exposure or CL treatment. In contrast, uptake of lipoprotein-derived fatty acids into chronically activated thermogenic adipose tissues was substantially increased in UCP1-deficient mice. This effect is linked to higher sympathetic tone in adipose tissues of Ucp1(-/-) mice, as indicated by elevated levels of thermogenic genes in BAT and WAT. Thus, glucose and lipoprotein handling does not necessarily reflect UCP1-dependent thermogenic activity, but especially lipid uptake rather mirrors sympathetic activation of adipose tissues.
Original language | English |
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Journal | Journal of Lipid Research |
Volume | 61 |
Issue number | 11 |
Pages (from-to) | 1377-1389 |
Number of pages | 13 |
ISSN | 0022-2275 |
DOIs | |
Publication status | Published - 2020 |
Externally published | Yes |
- adipocytes, lipase, lipoprotein, lipid metabolism, hormone-sensitive, lipid transport, lipolysis and fatty acid metabolism, lipoprotein metabolism, triglycerides, MUSCLE-BASED THERMOGENESIS, DIET-INDUCED THERMOGENESIS, FATTY-ACID TRANSPORT, ENERGY-EXPENDITURE, NONSHIVERING THERMOGENESIS, OXIDATIVE-METABOLISM, COLD-EXPOSURE, UCP1, WHITE, FGF21
Research areas
ID: 251945394