Describing the fecal metabolome in cryogenically collected samples from healthy participants

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Describing the fecal metabolome in cryogenically collected samples from healthy participants. / Trošt, Kajetan; Ahonen, Linda; Suvitaival, Tommi; Christiansen, Nina; Nielsen, Trine; Thiele, Maja; Jacobsen, Suganya; Krag, Aleksander; Rossing, Peter; Hansen, Torben; Dragsted, Lars Ove; Legido-Quigley, Cristina.

In: Scientific Reports, Vol. 10, No. 1, 885, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Trošt, K, Ahonen, L, Suvitaival, T, Christiansen, N, Nielsen, T, Thiele, M, Jacobsen, S, Krag, A, Rossing, P, Hansen, T, Dragsted, LO & Legido-Quigley, C 2020, 'Describing the fecal metabolome in cryogenically collected samples from healthy participants', Scientific Reports, vol. 10, no. 1, 885. https://doi.org/10.1038/s41598-020-57888-w

APA

Trošt, K., Ahonen, L., Suvitaival, T., Christiansen, N., Nielsen, T., Thiele, M., Jacobsen, S., Krag, A., Rossing, P., Hansen, T., Dragsted, L. O., & Legido-Quigley, C. (2020). Describing the fecal metabolome in cryogenically collected samples from healthy participants. Scientific Reports, 10(1), [885]. https://doi.org/10.1038/s41598-020-57888-w

Vancouver

Trošt K, Ahonen L, Suvitaival T, Christiansen N, Nielsen T, Thiele M et al. Describing the fecal metabolome in cryogenically collected samples from healthy participants. Scientific Reports. 2020;10(1). 885. https://doi.org/10.1038/s41598-020-57888-w

Author

Trošt, Kajetan ; Ahonen, Linda ; Suvitaival, Tommi ; Christiansen, Nina ; Nielsen, Trine ; Thiele, Maja ; Jacobsen, Suganya ; Krag, Aleksander ; Rossing, Peter ; Hansen, Torben ; Dragsted, Lars Ove ; Legido-Quigley, Cristina. / Describing the fecal metabolome in cryogenically collected samples from healthy participants. In: Scientific Reports. 2020 ; Vol. 10, No. 1.

Bibtex

@article{b9db548850614493a019d1c9cfb0f387,
title = "Describing the fecal metabolome in cryogenically collected samples from healthy participants",
abstract = "The chemical composition of feces plays an important role in human metabolism. Metabolomics and lipidomics are valuable tools for screening the metabolite composition in feces. Here we set out to describe fecal metabolite composition in healthy participants in frozen stools. Frozen stool samples were collected from 10 healthy volunteers and cryogenically drilled in four areas along the specimen. Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatography and time of flight mass spectrometry. Lipids were detected using ultra high-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. 2326 metabolic features were detected. Out of a total of 298 metabolites that were annotated we report here 185 that showed a technical variation of x < 30%. These metabolites included amino acids, fatty acid derivatives, carboxylic acids and phenolic compounds. Lipids predominantly belonged to the groups of diacylglycerols, triacylglycerols and ceramides. Metabolites varied between sampling areas, some were broadly homogeneous, others varied 80%. A LASSO-computed network using metabolites present in all areas showed two main clusters describing the system, DAG lipids and phenyllactic acid. In feces from healthy participants, the main groups detected were phenolic compounds, ceramides, diacylglycerols and triacylglycerols.",
author = "Kajetan Tro{\v s}t and Linda Ahonen and Tommi Suvitaival and Nina Christiansen and Trine Nielsen and Maja Thiele and Suganya Jacobsen and Aleksander Krag and Peter Rossing and Torben Hansen and Dragsted, {Lars Ove} and Cristina Legido-Quigley",
note = "CURIS 2020 NEXS 033",
year = "2020",
doi = "10.1038/s41598-020-57888-w",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Describing the fecal metabolome in cryogenically collected samples from healthy participants

AU - Trošt, Kajetan

AU - Ahonen, Linda

AU - Suvitaival, Tommi

AU - Christiansen, Nina

AU - Nielsen, Trine

AU - Thiele, Maja

AU - Jacobsen, Suganya

AU - Krag, Aleksander

AU - Rossing, Peter

AU - Hansen, Torben

AU - Dragsted, Lars Ove

AU - Legido-Quigley, Cristina

N1 - CURIS 2020 NEXS 033

PY - 2020

Y1 - 2020

N2 - The chemical composition of feces plays an important role in human metabolism. Metabolomics and lipidomics are valuable tools for screening the metabolite composition in feces. Here we set out to describe fecal metabolite composition in healthy participants in frozen stools. Frozen stool samples were collected from 10 healthy volunteers and cryogenically drilled in four areas along the specimen. Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatography and time of flight mass spectrometry. Lipids were detected using ultra high-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. 2326 metabolic features were detected. Out of a total of 298 metabolites that were annotated we report here 185 that showed a technical variation of x < 30%. These metabolites included amino acids, fatty acid derivatives, carboxylic acids and phenolic compounds. Lipids predominantly belonged to the groups of diacylglycerols, triacylglycerols and ceramides. Metabolites varied between sampling areas, some were broadly homogeneous, others varied 80%. A LASSO-computed network using metabolites present in all areas showed two main clusters describing the system, DAG lipids and phenyllactic acid. In feces from healthy participants, the main groups detected were phenolic compounds, ceramides, diacylglycerols and triacylglycerols.

AB - The chemical composition of feces plays an important role in human metabolism. Metabolomics and lipidomics are valuable tools for screening the metabolite composition in feces. Here we set out to describe fecal metabolite composition in healthy participants in frozen stools. Frozen stool samples were collected from 10 healthy volunteers and cryogenically drilled in four areas along the specimen. Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatography and time of flight mass spectrometry. Lipids were detected using ultra high-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. 2326 metabolic features were detected. Out of a total of 298 metabolites that were annotated we report here 185 that showed a technical variation of x < 30%. These metabolites included amino acids, fatty acid derivatives, carboxylic acids and phenolic compounds. Lipids predominantly belonged to the groups of diacylglycerols, triacylglycerols and ceramides. Metabolites varied between sampling areas, some were broadly homogeneous, others varied 80%. A LASSO-computed network using metabolites present in all areas showed two main clusters describing the system, DAG lipids and phenyllactic acid. In feces from healthy participants, the main groups detected were phenolic compounds, ceramides, diacylglycerols and triacylglycerols.

U2 - 10.1038/s41598-020-57888-w

DO - 10.1038/s41598-020-57888-w

M3 - Journal article

C2 - 31965056

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 885

ER -

ID: 237655136