Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans: A Randomized Double-Blind Placebo-Controlled Trial
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans : A Randomized Double-Blind Placebo-Controlled Trial. / Reijnders, Dorien; Goossens, Gijs H; Hermes, Gerben D A; Neis, Evelien P J G; van der Beek, Christina M; Most, Jasper; Holst, Jens J; Lenaerts, Kaatje; Kootte, Ruud S; Nieuwdorp, Max; Groen, Albert K; Olde Damink, Steven W M; Boekschoten, Mark V; Smidt, Hauke; Zoetendal, Erwin G; Dejong, Cornelis H C; Blaak, Ellen E.
In: Cell Metabolism, Vol. 24, No. 1, 12.07.2016, p. 63-74.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Effects of Gut Microbiota Manipulation by Antibiotics on Host Metabolism in Obese Humans
T2 - A Randomized Double-Blind Placebo-Controlled Trial
AU - Reijnders, Dorien
AU - Goossens, Gijs H
AU - Hermes, Gerben D A
AU - Neis, Evelien P J G
AU - van der Beek, Christina M
AU - Most, Jasper
AU - Holst, Jens J
AU - Lenaerts, Kaatje
AU - Kootte, Ruud S
AU - Nieuwdorp, Max
AU - Groen, Albert K
AU - Olde Damink, Steven W M
AU - Boekschoten, Mark V
AU - Smidt, Hauke
AU - Zoetendal, Erwin G
AU - Dejong, Cornelis H C
AU - Blaak, Ellen E
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/7/12
Y1 - 2016/7/12
N2 - The gut microbiota has been implicated in obesity and cardiometabolic diseases, although evidence in humans is scarce. We investigated how gut microbiota manipulation by antibiotics (7-day administration of amoxicillin, vancomycin, or placebo) affects host metabolism in 57 obese, prediabetic men. Vancomycin, but not amoxicillin, decreased bacterial diversity and reduced Firmicutes involved in short-chain fatty acid and bile acid metabolism, concomitant with altered plasma and/or fecal metabolite concentrations. Adipose tissue gene expression of oxidative pathways was upregulated by antibiotics, whereas immune-related pathways were downregulated by vancomycin. Antibiotics did not affect tissue-specific insulin sensitivity, energy/substrate metabolism, postprandial hormones and metabolites, systemic inflammation, gut permeability, and adipocyte size. Importantly, energy harvest, adipocyte size, and whole-body insulin sensitivity were not altered at 8-week follow-up, despite a still considerably altered microbial composition, indicating that interference with adult microbiota by 7-day antibiotic treatment has no clinically relevant impact on metabolic health in obese humans.
AB - The gut microbiota has been implicated in obesity and cardiometabolic diseases, although evidence in humans is scarce. We investigated how gut microbiota manipulation by antibiotics (7-day administration of amoxicillin, vancomycin, or placebo) affects host metabolism in 57 obese, prediabetic men. Vancomycin, but not amoxicillin, decreased bacterial diversity and reduced Firmicutes involved in short-chain fatty acid and bile acid metabolism, concomitant with altered plasma and/or fecal metabolite concentrations. Adipose tissue gene expression of oxidative pathways was upregulated by antibiotics, whereas immune-related pathways were downregulated by vancomycin. Antibiotics did not affect tissue-specific insulin sensitivity, energy/substrate metabolism, postprandial hormones and metabolites, systemic inflammation, gut permeability, and adipocyte size. Importantly, energy harvest, adipocyte size, and whole-body insulin sensitivity were not altered at 8-week follow-up, despite a still considerably altered microbial composition, indicating that interference with adult microbiota by 7-day antibiotic treatment has no clinically relevant impact on metabolic health in obese humans.
KW - Journal Article
U2 - 10.1016/j.cmet.2016.06.016
DO - 10.1016/j.cmet.2016.06.016
M3 - Journal article
C2 - 27411009
VL - 24
SP - 63
EP - 74
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 1
ER -
ID: 165936225