JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes

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JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes. / Negoita, Florentina; Vavakova, Magdalena; Saell, Johanna; Laurencikiene, Jurga; Goeransson, Olga.

In: Cellular Signalling, Vol. 76, 109786, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Negoita, F, Vavakova, M, Saell, J, Laurencikiene, J & Goeransson, O 2020, 'JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes', Cellular Signalling, vol. 76, 109786. https://doi.org/10.1016/j.cellsig.2020.109786

APA

Negoita, F., Vavakova, M., Saell, J., Laurencikiene, J., & Goeransson, O. (2020). JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes. Cellular Signalling, 76, [109786]. https://doi.org/10.1016/j.cellsig.2020.109786

Vancouver

Negoita F, Vavakova M, Saell J, Laurencikiene J, Goeransson O. JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes. Cellular Signalling. 2020;76. 109786. https://doi.org/10.1016/j.cellsig.2020.109786

Author

Negoita, Florentina ; Vavakova, Magdalena ; Saell, Johanna ; Laurencikiene, Jurga ; Goeransson, Olga. / JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes. In: Cellular Signalling. 2020 ; Vol. 76.

Bibtex

@article{0d71a04203a944e2b18f741514d758a2,
title = "JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes",
abstract = "Background: Salt-inducible kinase 2 (SIK2) is abundant in adipocytes, but downregulated in adipose tissue from individuals with obesity and insulin resistance. Moreover, SIK isoforms are required for normal insulin signalling and glucose uptake in adipocytes, but the underlying molecular mechanisms are currently not known. The adherens junction protein JUP, also termed plakoglobin or gamma-catenin, has recently been reported to promote insulin signalling in muscle cells.Objective: The objective of this study was to analyse if JUP is required for insulin signalling in adipocytes and the underlying molecular mechanisms of this regulation.Methods: Co-expression of SIK2 and JUP mRNA levels in adipose tissue from a human cohort was analysed. siRNA silencing and/or pharmacological inhibition of SIK2, JUP, class IIa HDACs and CRTC2 was employed in 3T3-L1- and primary rat adipocytes. JUP protein expression was analysed by western blot and mRNA levels by qPCR. Insulin signalling was evaluated by western blot as levels of phosphorylated PKB/Akt and AS160, and by monitoring the uptake of H-3-2-deoxyglucose.Results: mRNA expression of SIK2 correlated with that of JUP in human adipose tissue. SIK2 inhibition or si-lencing resulted in downregulation of JUP mRNA and protein expression in 3T3 -L1and in primary rat adipocytes. Moreover, JUP silencing reduced the expression of PKB and the downstream substrate AS160, and consequently attenuated activity in the insulin signalling pathway, including insulin-induced glucose uptake. The known SIK2 substrates CRTC2 and class IIa HDACs were found to play a role in the SIK-mediated regulation of JUP expression.Conclusions: These findings identify JUP as a novel player in the regulation of insulin sensitivity in adipocytes, and suggest that changes in JUP expression could contribute to the effect of SIK2 on insulin signalling in these cells.",
keywords = "SIK2, JUP, HDAC, CRTC2, Insulin signalling, Adipocytes, BETA-CATENIN, ADIPOSE-TISSUE, PHOSPHORYLATION, ADIPOGENESIS, PLAKOGLOBIN, INHIBITION, EXPRESSION, PROMOTES, MUSCLE",
author = "Florentina Negoita and Magdalena Vavakova and Johanna Saell and Jurga Laurencikiene and Olga Goeransson",
year = "2020",
doi = "10.1016/j.cellsig.2020.109786",
language = "English",
volume = "76",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - JUP/plakoglobin is regulated by salt-inducible kinase 2, and is required for insulin-induced signalling and glucose uptake in adipocytes

AU - Negoita, Florentina

AU - Vavakova, Magdalena

AU - Saell, Johanna

AU - Laurencikiene, Jurga

AU - Goeransson, Olga

PY - 2020

Y1 - 2020

N2 - Background: Salt-inducible kinase 2 (SIK2) is abundant in adipocytes, but downregulated in adipose tissue from individuals with obesity and insulin resistance. Moreover, SIK isoforms are required for normal insulin signalling and glucose uptake in adipocytes, but the underlying molecular mechanisms are currently not known. The adherens junction protein JUP, also termed plakoglobin or gamma-catenin, has recently been reported to promote insulin signalling in muscle cells.Objective: The objective of this study was to analyse if JUP is required for insulin signalling in adipocytes and the underlying molecular mechanisms of this regulation.Methods: Co-expression of SIK2 and JUP mRNA levels in adipose tissue from a human cohort was analysed. siRNA silencing and/or pharmacological inhibition of SIK2, JUP, class IIa HDACs and CRTC2 was employed in 3T3-L1- and primary rat adipocytes. JUP protein expression was analysed by western blot and mRNA levels by qPCR. Insulin signalling was evaluated by western blot as levels of phosphorylated PKB/Akt and AS160, and by monitoring the uptake of H-3-2-deoxyglucose.Results: mRNA expression of SIK2 correlated with that of JUP in human adipose tissue. SIK2 inhibition or si-lencing resulted in downregulation of JUP mRNA and protein expression in 3T3 -L1and in primary rat adipocytes. Moreover, JUP silencing reduced the expression of PKB and the downstream substrate AS160, and consequently attenuated activity in the insulin signalling pathway, including insulin-induced glucose uptake. The known SIK2 substrates CRTC2 and class IIa HDACs were found to play a role in the SIK-mediated regulation of JUP expression.Conclusions: These findings identify JUP as a novel player in the regulation of insulin sensitivity in adipocytes, and suggest that changes in JUP expression could contribute to the effect of SIK2 on insulin signalling in these cells.

AB - Background: Salt-inducible kinase 2 (SIK2) is abundant in adipocytes, but downregulated in adipose tissue from individuals with obesity and insulin resistance. Moreover, SIK isoforms are required for normal insulin signalling and glucose uptake in adipocytes, but the underlying molecular mechanisms are currently not known. The adherens junction protein JUP, also termed plakoglobin or gamma-catenin, has recently been reported to promote insulin signalling in muscle cells.Objective: The objective of this study was to analyse if JUP is required for insulin signalling in adipocytes and the underlying molecular mechanisms of this regulation.Methods: Co-expression of SIK2 and JUP mRNA levels in adipose tissue from a human cohort was analysed. siRNA silencing and/or pharmacological inhibition of SIK2, JUP, class IIa HDACs and CRTC2 was employed in 3T3-L1- and primary rat adipocytes. JUP protein expression was analysed by western blot and mRNA levels by qPCR. Insulin signalling was evaluated by western blot as levels of phosphorylated PKB/Akt and AS160, and by monitoring the uptake of H-3-2-deoxyglucose.Results: mRNA expression of SIK2 correlated with that of JUP in human adipose tissue. SIK2 inhibition or si-lencing resulted in downregulation of JUP mRNA and protein expression in 3T3 -L1and in primary rat adipocytes. Moreover, JUP silencing reduced the expression of PKB and the downstream substrate AS160, and consequently attenuated activity in the insulin signalling pathway, including insulin-induced glucose uptake. The known SIK2 substrates CRTC2 and class IIa HDACs were found to play a role in the SIK-mediated regulation of JUP expression.Conclusions: These findings identify JUP as a novel player in the regulation of insulin sensitivity in adipocytes, and suggest that changes in JUP expression could contribute to the effect of SIK2 on insulin signalling in these cells.

KW - SIK2

KW - JUP

KW - HDAC

KW - CRTC2

KW - Insulin signalling

KW - Adipocytes

KW - BETA-CATENIN

KW - ADIPOSE-TISSUE

KW - PHOSPHORYLATION

KW - ADIPOGENESIS

KW - PLAKOGLOBIN

KW - INHIBITION

KW - EXPRESSION

KW - PROMOTES

KW - MUSCLE

U2 - 10.1016/j.cellsig.2020.109786

DO - 10.1016/j.cellsig.2020.109786

M3 - Journal article

C2 - 32966883

VL - 76

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

M1 - 109786

ER -

ID: 252878779