Regulation of Dishevelled and β-catenin in rat skeletal muscle: An alternative exercise-induced GSK-3β signaling pathway
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Regulation of Dishevelled and β-catenin in rat skeletal muscle : An alternative exercise-induced GSK-3β signaling pathway. / Aschenbach, William G.; Ho, Richard C.; Sakamoto, Kei; Fujii, Nobuharu; Li, Yangfeng; Kim, Young Bum; Hirshman, Michael F.; Goodyear, Laurie J.
In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 291, No. 1, 17.07.2006, p. E152-E158.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regulation of Dishevelled and β-catenin in rat skeletal muscle
T2 - An alternative exercise-induced GSK-3β signaling pathway
AU - Aschenbach, William G.
AU - Ho, Richard C.
AU - Sakamoto, Kei
AU - Fujii, Nobuharu
AU - Li, Yangfeng
AU - Kim, Young Bum
AU - Hirshman, Michael F.
AU - Goodyear, Laurie J.
PY - 2006/7/17
Y1 - 2006/7/17
N2 - β-catenin is a multifunctional protein involved in cell-cell adhesion and the Wnt signaling pathway. β-Catenin is activated upon its dephosphorylation, an event triggered by Dishevelled (Dvl)-mediated phosphorylation and deactivation of glycogen synthase kinase-3β (GSK-3β). In skeletal muscle, both insulin and exercise decrease GSK-3β activity, and we tested the hypothesis that these two stimuli regulate β-catenin. Immunoblotting demonstrated that Dvl, Axin, GSK-3β, and β-catenin proteins are expressed in rat red and white gastrocnemius muscles. Treadmill running exercise in vivo significantly decreased β-catenin phosphorylation in both muscle types, with complete dephosphorylation being elicited by maximal exercise. β-Catenin dephosphorylation was intensity dependent, as dephosphorylation was highly correlated with muscle glycogen depletion during exercise (r2 = 0.84, P < 0.001). β-Catenin dephosphorylation was accompanied by increases in GSK-3β Ser9 phosphorylation and Dvl-GSK-3β association. In contrast to exercise, maximal insulin treatment (1 U/kg body wt) had no effect on skeletal muscle β-catenin phosphorylation or Dvl-GSK-3β interaction. In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3β in skeletal muscle, an event paralleled by β-catenin dephosphorylation.
AB - β-catenin is a multifunctional protein involved in cell-cell adhesion and the Wnt signaling pathway. β-Catenin is activated upon its dephosphorylation, an event triggered by Dishevelled (Dvl)-mediated phosphorylation and deactivation of glycogen synthase kinase-3β (GSK-3β). In skeletal muscle, both insulin and exercise decrease GSK-3β activity, and we tested the hypothesis that these two stimuli regulate β-catenin. Immunoblotting demonstrated that Dvl, Axin, GSK-3β, and β-catenin proteins are expressed in rat red and white gastrocnemius muscles. Treadmill running exercise in vivo significantly decreased β-catenin phosphorylation in both muscle types, with complete dephosphorylation being elicited by maximal exercise. β-Catenin dephosphorylation was intensity dependent, as dephosphorylation was highly correlated with muscle glycogen depletion during exercise (r2 = 0.84, P < 0.001). β-Catenin dephosphorylation was accompanied by increases in GSK-3β Ser9 phosphorylation and Dvl-GSK-3β association. In contrast to exercise, maximal insulin treatment (1 U/kg body wt) had no effect on skeletal muscle β-catenin phosphorylation or Dvl-GSK-3β interaction. In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3β in skeletal muscle, an event paralleled by β-catenin dephosphorylation.
KW - Akt
KW - Glycogen-synthase kinase-3β
KW - Insulin
KW - Protein kinase C
KW - Wnt
UR - http://www.scopus.com/inward/record.url?scp=33745857968&partnerID=8YFLogxK
U2 - 10.1152/ajpendo.00180.2005
DO - 10.1152/ajpendo.00180.2005
M3 - Journal article
C2 - 16478782
AN - SCOPUS:33745857968
VL - 291
SP - E152-E158
JO - A J P: Endocrinology and Metabolism (Online)
JF - A J P: Endocrinology and Metabolism (Online)
SN - 1522-1555
IS - 1
ER -
ID: 239585079