The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver

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The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. / Patel, Kashyap; Foretz, Marc; Marion, Allison; Campbell, David G.; Gourlay, Robert; Boudaba, Nadia; Tournier, Emilie; Titchenell, Paul; Peggie, Mark; Deak, Maria; Wan, Min; Kaestner, Klaus H.; Göransson, Olga; Viollet, Benoit; Gray, Nathanael S.; Birnbaum, Morris J.; Sutherland, Calum; Sakamoto, Kei.

In: Nature Communications, Vol. 5, 4535, 04.08.2014.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Patel, K, Foretz, M, Marion, A, Campbell, DG, Gourlay, R, Boudaba, N, Tournier, E, Titchenell, P, Peggie, M, Deak, M, Wan, M, Kaestner, KH, Göransson, O, Viollet, B, Gray, NS, Birnbaum, MJ, Sutherland, C & Sakamoto, K 2014, 'The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver', Nature Communications, vol. 5, 4535. https://doi.org/10.1038/ncomms5535

APA

Patel, K., Foretz, M., Marion, A., Campbell, D. G., Gourlay, R., Boudaba, N., Tournier, E., Titchenell, P., Peggie, M., Deak, M., Wan, M., Kaestner, K. H., Göransson, O., Viollet, B., Gray, N. S., Birnbaum, M. J., Sutherland, C., & Sakamoto, K. (2014). The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. Nature Communications, 5, [4535]. https://doi.org/10.1038/ncomms5535

Vancouver

Patel K, Foretz M, Marion A, Campbell DG, Gourlay R, Boudaba N et al. The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. Nature Communications. 2014 Aug 4;5. 4535. https://doi.org/10.1038/ncomms5535

Author

Patel, Kashyap ; Foretz, Marc ; Marion, Allison ; Campbell, David G. ; Gourlay, Robert ; Boudaba, Nadia ; Tournier, Emilie ; Titchenell, Paul ; Peggie, Mark ; Deak, Maria ; Wan, Min ; Kaestner, Klaus H. ; Göransson, Olga ; Viollet, Benoit ; Gray, Nathanael S. ; Birnbaum, Morris J. ; Sutherland, Calum ; Sakamoto, Kei. / The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. In: Nature Communications. 2014 ; Vol. 5.

Bibtex

@article{1de0c0f6736b47f6adddf7066286c9c2,
title = "The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver",
abstract = "LKB1 is a master kinase that regulates metabolism and growth through adenosine monophosphate-activated protein kinase (AMPK) and 12 other closely related kinases. Liver-specific ablation of LKB1 causes increased glucose production in hepatocytes in vitro and hyperglycaemia in fasting mice in vivo. Here we report that the salt-inducible kinases (SIK1, 2 and 3), members of the AMPK-related kinase family, play a key role as gluconeogenic suppressors downstream of LKB1 in the liver. The selective SIK inhibitor HG-9-91-01 promotes dephosphorylation of transcriptional co-activators CRTC2/3 resulting in enhanced gluconeogenic gene expression and glucose production in hepatocytes, an effect that is abolished when an HG-9-91-01-insensitive mutant SIK is introduced or LKB1 is ablated. Although SIK2 was proposed as a key regulator of insulin-mediated suppression of gluconeogenesis, we provide genetic evidence that liver-specific ablation of SIK2 alone has no effect on gluconeogenesis and insulin does not modulate SIK2 phosphorylation or activity. Collectively, we demonstrate that the LKB1-SIK pathway functions as a key gluconeogenic gatekeeper in the liver.",
author = "Kashyap Patel and Marc Foretz and Allison Marion and Campbell, {David G.} and Robert Gourlay and Nadia Boudaba and Emilie Tournier and Paul Titchenell and Mark Peggie and Maria Deak and Min Wan and Kaestner, {Klaus H.} and Olga G{\"o}ransson and Benoit Viollet and Gray, {Nathanael S.} and Birnbaum, {Morris J.} and Calum Sutherland and Kei Sakamoto",
year = "2014",
month = aug,
day = "4",
doi = "10.1038/ncomms5535",
language = "English",
volume = "5",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver

AU - Patel, Kashyap

AU - Foretz, Marc

AU - Marion, Allison

AU - Campbell, David G.

AU - Gourlay, Robert

AU - Boudaba, Nadia

AU - Tournier, Emilie

AU - Titchenell, Paul

AU - Peggie, Mark

AU - Deak, Maria

AU - Wan, Min

AU - Kaestner, Klaus H.

AU - Göransson, Olga

AU - Viollet, Benoit

AU - Gray, Nathanael S.

AU - Birnbaum, Morris J.

AU - Sutherland, Calum

AU - Sakamoto, Kei

PY - 2014/8/4

Y1 - 2014/8/4

N2 - LKB1 is a master kinase that regulates metabolism and growth through adenosine monophosphate-activated protein kinase (AMPK) and 12 other closely related kinases. Liver-specific ablation of LKB1 causes increased glucose production in hepatocytes in vitro and hyperglycaemia in fasting mice in vivo. Here we report that the salt-inducible kinases (SIK1, 2 and 3), members of the AMPK-related kinase family, play a key role as gluconeogenic suppressors downstream of LKB1 in the liver. The selective SIK inhibitor HG-9-91-01 promotes dephosphorylation of transcriptional co-activators CRTC2/3 resulting in enhanced gluconeogenic gene expression and glucose production in hepatocytes, an effect that is abolished when an HG-9-91-01-insensitive mutant SIK is introduced or LKB1 is ablated. Although SIK2 was proposed as a key regulator of insulin-mediated suppression of gluconeogenesis, we provide genetic evidence that liver-specific ablation of SIK2 alone has no effect on gluconeogenesis and insulin does not modulate SIK2 phosphorylation or activity. Collectively, we demonstrate that the LKB1-SIK pathway functions as a key gluconeogenic gatekeeper in the liver.

AB - LKB1 is a master kinase that regulates metabolism and growth through adenosine monophosphate-activated protein kinase (AMPK) and 12 other closely related kinases. Liver-specific ablation of LKB1 causes increased glucose production in hepatocytes in vitro and hyperglycaemia in fasting mice in vivo. Here we report that the salt-inducible kinases (SIK1, 2 and 3), members of the AMPK-related kinase family, play a key role as gluconeogenic suppressors downstream of LKB1 in the liver. The selective SIK inhibitor HG-9-91-01 promotes dephosphorylation of transcriptional co-activators CRTC2/3 resulting in enhanced gluconeogenic gene expression and glucose production in hepatocytes, an effect that is abolished when an HG-9-91-01-insensitive mutant SIK is introduced or LKB1 is ablated. Although SIK2 was proposed as a key regulator of insulin-mediated suppression of gluconeogenesis, we provide genetic evidence that liver-specific ablation of SIK2 alone has no effect on gluconeogenesis and insulin does not modulate SIK2 phosphorylation or activity. Collectively, we demonstrate that the LKB1-SIK pathway functions as a key gluconeogenic gatekeeper in the liver.

UR - http://www.scopus.com/inward/record.url?scp=84905457029&partnerID=8YFLogxK

U2 - 10.1038/ncomms5535

DO - 10.1038/ncomms5535

M3 - Journal article

C2 - 25088745

AN - SCOPUS:84905457029

VL - 5

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 4535

ER -

ID: 239213063